Abstract:

In order to develop a novel particle pre-culture spawn of Agaricus bisporus with high mycelia content,strong mycelia vitality,more germination points and loose uniformity,single factor and orthogonal tests were adapted and the relative amount of mycelium was used as an index to investigate the effects of C/N,initial pH,water content and the added amount of perlite on the novel particle pre-culture spawn of A. bisporus,and then the formula was optimized. Then we investigated the effect of propagation of the novel particle pre-culture spawn of A. bisporus by the comparison of multiple indexes while using the grain pre-culture spawn as control. The results showed that the lower the C/N content and the less perlite of A. bisporus granule matrix formula were added,the higher the relative mycelia quantity was detected. The optimal formula of the novel particle pre-culture spawn was A₁B₁C₃D₃,constituted by rapeseed meal 78 g,wheat bran 10 g,secondary compost 20 g,diatomite 20 g,perlite 5 g,and light calcium carbonate 15 g. The initial pH was 7.6,and water content was 54%. The particle number of the novel particle pre-culture spawn was 280-300 particles/g,which was more than that of millet pre-culture spawn 180-200 particles/g and significantly more than that of wheat pre-culture spawn 16-18 particles/g. The relative mycelia of the novel particle pre-culture spawn was 326.5 mg/g. It was 1.29 times of 252.9 mg/g of millet pre-culture spawn and 2.35 times of 139.2 mg/g of wheat pre-culture spawn,respectively. When the inoculation amount was 0.2%,the average bag-filled time of the novel particle pre-culture spawn was 15.8 d,which was shorter than 18.4 d of millet pre-culture spawn and 19.6 d of wheat pre-culture spawn. It indicates that the novel particle pre-culture spawn had advantages over grain pre-culture for propagating spawn of A. bisporus.

Key words: Agaricus bisporus, pre-culture spawn, particle strain, formulation optimization