Biotechnology Bulletin ›› 2021, Vol. 37 ›› Issue (11): 125-133.doi: 10.13560/j.cnki.biotech.bull.1985.2021-1011

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Screening of Superior Strains Isolated from Wild Cordyceps militaris in Mountain Tai

GUO Yu-feng(), WANG Qiao, WANG Xiang-feng, ZHOU Ting, YANG Xiao-jun, WANG Qing-ji, WANG Li()   

  1. Shandong Provincial Key Laboratory of Agricultural Microbiology,College of Plant Protection,Shandong Agricultural University,Tai’an 271018
  • Received:2021-08-12 Online:2021-11-26 Published:2021-12-03
  • Contact: WANG Li E-mail:yufeng_12021@163.com;haoyou0102@163.com

Abstract:

In order to further dig the resources of Cordyceps militaris in Mountain Tai area and to obtain the superior strains of C. militaris with high yield and high quality,14 wild strains isolated from wild C. militaris in Mountain Tai were used as experimental materials,the mycelial growth rate,colony characteristics,fruiting body morphology and yield,and the main active substances(cordycepin,N6-(2-hydroxyethyl)adenosine,polysaccharide,β-carotenoid,and lutein)were determined,and the characteristics of each strain were evaluated by the normalization method. Finally,2 strains with superior comprehensive characteristics and 1 strain with high cordycepin content were selected. The selected two superior strains had the best comprehensive quality,the cultivation periods of fruit body were both 46.3 d,fruit bodies were thick and long,the biological efficiency was 55.31% and 58.08%,the cordycepin content was 5.43 mg/g and 7.13 mg/g,the adenosine content was 0.38 mg/g and 0.56 mg/g,the polysaccharide content was 90.44 mg/g and 84.68 mg/g,respectively. The high cordycepin content strain had lower yield,but the cordycepin content was up to 15.14 mg/g and adenosine content was also high(0.66 mg/g).

Key words: Cordyceps militaris, screening of strains, active substance