Product Analysis of Degrading Aflatoxin B1 by a Strain Bacillus subtilis

doi:10.13560/j.cnki.biotech.bull.1985.2021-0179

Abstract

Abstract:

This work aims to enrich the resources of biodegradable aflatoxin B₁ and study the degradation mechanism and toxicity of the product. Triple quadrupole liquid chromatography-tandem mass spectrometry（UPLC-MS/MS）and ¹H NMR were combined to detect the degraded products of aflatoxin B₁（AFB₁）by a strain Bacillus subtilis WTX1,and the cell toxicology tests on the degradation products were performed. The degrading mechanism of AFB₁ by the strain WTX1 was to destroy the structures of furan ring dihydrogen double bond,vanillin aliphatic ring and pentenone ring to form small molecule compounds and to achieve detoxification effect. The cell toxicology test confirmed that the degraded products by strain WTX1 was basically non-toxic to cells. The degradation mechanism of strain WTX1 is to destroy the toxicity and mutation-causing structure of AFB1 and to produce small molecule compounds. The product of degraded AFB₁ by WTX1 is not mutagenic and toxic to cells. The later study of the main role of substances from strain degrading AFB1 would provide a new idea for industrial removal of AFB₁ toxin.

Key words: aflatoxin B₁, Bacillus subtilis, product analysis, cell toxicology

TANG Ying, HUANG Jia, DENG Zhan-rui, YANG Xiao-nan. Product Analysis of Degrading Aflatoxin B₁ by a Strain Bacillus subtilis[J]. Biotechnology Bulletin, 2021, 37(12): 82-90.

Figures/Tables 8

References 27

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培养时间Incubation time	组别 Group	Viable Cell Density （×10⁶ cells/mL）±RSD	Average date for double tine±RSD/h	Average date for genration number	P
0 d	参照组	0.010±0.021	/	/	/
	试验组	0.010±0.016	/	/	/
	对照组	0.010±0.020	/	/	/
1 d	参照组	0.029±013	21.609±0.74	1.362	>0.05
	试验组	0.022±0.023	21.920±0.98	1.293	>0.05
	对照组	0.013±0.031	45.270±1.20	0.821	<0.05
2 d	参照组	0.063±0.016	22.937±0.72	2.654	>0.05
	试验组	0.053±0.022	22.603±0.46	2.437	>0.05
	对照组	0.008±0.035	57.558±1.03	0.629	<0.05
3 d	参照组	0.131±0.031	21.193±0.59	3.815	>0.05
	试验组	0.116±0.027	22.462±0.91	3.623	>0.05
	对照组	0.003±0.010	68.361±1.31	0.231	<0.05

培养时间Incubation time	组别 Group	Viable Cell Density （×10⁶ cells/mL）±RSD	Average date for double tine±RSD/h	Average date for genration number	P
0 d	参照组	0.010±0.021	/	/	/
	试验组	0.010±0.016	/	/	/
	对照组	0.010±0.020	/	/	/
1 d	参照组	0.029±013	21.609±0.74	1.362	>0.05
	试验组	0.022±0.023	21.920±0.98	1.293	>0.05
	对照组	0.013±0.031	45.270±1.20	0.821	<0.05
2 d	参照组	0.063±0.016	22.937±0.72	2.654	>0.05
	试验组	0.053±0.022	22.603±0.46	2.437	>0.05
	对照组	0.008±0.035	57.558±1.03	0.629	<0.05
3 d	参照组	0.131±0.031	21.193±0.59	3.815	>0.05
	试验组	0.116±0.027	22.462±0.91	3.623	>0.05
	对照组	0.003±0.010	68.361±1.31	0.231	<0.05

Product Analysis of Degrading Aflatoxin B₁ by a Strain Bacillus subtilis

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References 27

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