Biotechnology Bulletin ›› 2021, Vol. 37 ›› Issue (2): 15-23.doi: 10.13560/j.cnki.biotech.bull.1985.2020-0774

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Isolation and Characterization of Two New GLABROUS1 Alleles in Arabidopsis

ZHENG Ye-zi(), WANG Dan, PAN Mi, WANG Yan-ling, AN Li-jun()   

  1. 1. State Key Laboratory of Crop Stress Biology for Arid Areas,Northwest A&F University,Yangling 712100
    2. College of Life Sciences,Northwest A&F University,Yangling 712100
  • Received:2020-06-24 Online:2021-02-26 Published:2021-02-26
  • Contact: AN Li-jun E-mail:463785896@qq.com;lijunan@nwsuaf.edu.cn

Abstract:

The studies on plant developmental biology has always been centered on uncovering the underlying mechanisms of cell fate determination. Trichome in model plant Arabidopsis is an elegant system to study the molecular mechanisms of several aspects including cell fate determination of plant. To identify and screen the new genetic factors controlling the trichome initiation of Arabidopsi,we carried out the large-scale forward genetic screening,and obtained two mutants f08-01 and vat002-07 in which the trichome of rosette leaves could not be formed or the number was significantly reduced. After cloning and genetic complementation of the mutation genes,we confirmed that these two mutants represented the new alleles of GLABROUS 1(GL1),which is known as a key regulator in trichome initiation. Previous studies suggested that the expression of GL1 itself might be tightly regulated during trichome development,but the underlying mechanisms are not clear yet. During our genetic complementation experiments,we found the 3' non-coding region of GL1 was vital for its function during trichome development. Further genetic and pharmacological analysis showed that the 3' non-coding sequence might be responsible for its mRNA stability and transcriptional activation.

Key words: trichome, GL1, mRNA-stability, cell fate determination, post-transcriptional regulation