Advances of Single-molecule Fluorescence Detection Techniques and Applications in Plant Biology

doi:10.13560/j.cnki.biotech.bull.1985.2021-0793

Abstract

Abstract:

Single-molecule fluorescence detection techniques（SMD）are used to image and track the conformational changes,dynamics and interactions of fluorescently labeled molecules at single-molecule level. Compared with the traditional molecular and genetic approaches,SMD can analyze the dynamical characteristics of individual molecules,especially instantaneous and occasionally events that may be hidden in massive average measurements. Therefore,SMD has been widely used in animal researches,which made numerous important contributions to molecular mechanisms in biology researches. However,the application of SMD in plants remains to be further developed. In this review,we introduced the most recently advances of SMD techniques. Then we summarized its application cases in plant biology,as well as the application prospect of SMD in plant biology researches in the near future.

Key words: single-molecule, fluorescence imaging, spectroscopy detection, plant biology

ZHANG Yuan, ZHANG Xue-ping, ZHANG Yue-qian, LI Xiao-juan. Advances of Single-molecule Fluorescence Detection Techniques and Applications in Plant Biology[J]. Biotechnology Bulletin, 2022, 38(1): 33-43.

Figures/Tables 2

Fig.1 Comparison of spatial and temporal resolution of various imaging techniques The left panel shows spatial resolution of various techniques. The x-axis is the axial resolution,and the y-axis is the lateral resolution of each imaging technique. The right panel shows the time resolution of various techniques. Temporal resolution is estimated based on the lowest exposure times required to image a single plane（SMLM,TIRF）or a volume of a typical mammalian cell（40 µm×40 µm×1 µm）with comparable signal-to-noise-ratio. Refer to Schermelleh et al[46]

Fig.2 Classification and advantages of different single-molecule detection techniques

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