Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (12): 287-299.doi: 10.13560/j.cnki.biotech.bull.1985.2022-0347

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Isolation, Identification, Degradation Characteristics and Metabolic Pathway of an Efficient Sodium Dodecyl Sulfate-degrading Bacterium

NIU Hong-yu1(), SHU Qian2, YANG Hai-jun1, YAN Zhi-yong1, TAN Ju2,3()   

  1. 1. School of Resources and Environment,Hunan Agricultural University,Changsha 410128
    2. School of Plant Protection,Hunan Agricultural University,Changsha 410128
    3. Changsha Ecological Environment Monitoring Center of Hunan Province,Changsha 410001
  • Received:2022-03-22 Online:2022-12-26 Published:2022-12-29
  • Contact: TAN Ju E-mail:nhy@stu.hunau.edu.cn;yhj@hunau.edu.cn

Abstract:

In order to obtain more abundant resources of sodium dodecyl sulfate(SDS)-degrading bacteria,a highly efficient SDS-degrading strain D2 with SDS as the only carbon source was isolated from the composite strain SDS1 screened and preserved in our laboratory. According to its morphological characteristics,physiological and biochemical experiments and 16S rRNA gene sequence molecular analysis,D2 was identified as Paraburkholderia tropica. The experimental results confirmed that the degradation rate of SDS by D2 increased to 98.3% at a temperature of 30℃,pH 7,salinity 0.1%(W/W)when the initial concentration of SDS was 1 200 mg/L,and an additional nitrogen source of sodium nitrate + ammonium chloride for 48 h. The main metabolites of the strain in SDS degradation were detected via ion chromatography,gas chromatography and liquid chromatography. The feasible degradation pathway of SDS by D2 might be SDS → sulfate +1-dodecanol → dodecanaldehyde → dodecanoic acid → acetic acid → CO2 + H2O. This study may provide microbial resources for the efficient degradation of SDS-containing surfactant wastewater.

Key words: sodium dodecyl sulfate, Paraburkholderia tropica, microbial degradation, isolation and identification, degradation characteristics, metabolism pathway