Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (3): 139-148.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0620
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TANG Bin(), LIU Wen-bin, LI Xiao-bo, WANG Ning, JIN Xiao-bao()
Received:
2021-05-11
Online:
2022-03-26
Published:
2022-04-06
Contact:
JIN Xiao-bao
E-mail:1375540061@qq.com;jinxf2001@163.com
TANG Bin, LIU Wen-bin, LI Xiao-bo, WANG Ning, JIN Xiao-bao. Screening and Identification of Strains Producing 7-β-xylosyltaxanes Glycoside Hydrolases from the Periplaneta americana Gut[J]. Biotechnology Bulletin, 2022, 38(3): 139-148.
Fig. 3 Fluorescence color image of xylanase-producing strain A to F:WA1-37,WA2-17,WA3-2-36,WA4-65,WA11-2-9 and WA14-2-7. The left is fluorescent color,and the right is self-control
菌株编号 Strain code | 菌落直径 Diameter of colony(d/cm) | 透明圈直径 Diameter of transparent circle of clony(D/cm) | 比值 Ratio(D/d) | ||
---|---|---|---|---|---|
内圈Inner circle | 外圈Outer circle | 内圈Inner circle | 外圈Outer circle | ||
1-37 | 0.75±0.02 | 1.67±0.12 | 3.68±0.18 | 2.21±0.11 | 4.88±0.12 |
2-17 | 0.83±0.05 | 1.54±0.05 | 2.66±0.04 | 1.87±0.16 | 3.23±0.17 |
3-2-36 | 0.64±0.06 | 1.33±0.07 | 2.89±0.02 | 2.13±0.06 | 4.63±0.17 |
4-65 | 0.68±0.08 | 1.72±0.12 | 3.50±0.17 | 2.56±0.16 | 5.03±0.13 |
11-2-9 | 0.62±0.04 | 1.33±0.05 | 3.72±0.04 | 2.07±0.12 | 5.55±0.17 |
14-2-7 | 0.62±0.04 | 1.50±0.03 | 2.92±0.04 | 2.44±0.10 | 4.80±0.15 |
Table 1 Colony diameters,Congo red transparent circles and their ratios of xylanase-producing strains(n=3)
菌株编号 Strain code | 菌落直径 Diameter of colony(d/cm) | 透明圈直径 Diameter of transparent circle of clony(D/cm) | 比值 Ratio(D/d) | ||
---|---|---|---|---|---|
内圈Inner circle | 外圈Outer circle | 内圈Inner circle | 外圈Outer circle | ||
1-37 | 0.75±0.02 | 1.67±0.12 | 3.68±0.18 | 2.21±0.11 | 4.88±0.12 |
2-17 | 0.83±0.05 | 1.54±0.05 | 2.66±0.04 | 1.87±0.16 | 3.23±0.17 |
3-2-36 | 0.64±0.06 | 1.33±0.07 | 2.89±0.02 | 2.13±0.06 | 4.63±0.17 |
4-65 | 0.68±0.08 | 1.72±0.12 | 3.50±0.17 | 2.56±0.16 | 5.03±0.13 |
11-2-9 | 0.62±0.04 | 1.33±0.05 | 3.72±0.04 | 2.07±0.12 | 5.55±0.17 |
14-2-7 | 0.62±0.04 | 1.50±0.03 | 2.92±0.04 | 2.44±0.10 | 4.80±0.15 |
Fig. 4 TLC result of transformed product of strain WA11-2-9 1st to 4th are:WA 10-1-2,WA 11-2-9,WA 11-1-3 and WA 23-1-5 transformation products,the points from 5th to 7th are:7-XDT,10-DAT and 10-DAB standards
Fig. 5 Morphological characteristics of strain WA11-2-9 A:The colony morphology of strain WA11-2-9. B:The gram staining diagram of strain WA11-2-9(100×)
检测项目Test item | 结果Result | 检测项目Test item | 结果Result | 检测项目Test item | 结果Result | ||
---|---|---|---|---|---|---|---|
淀粉水解 | + | H2S产生 | + | 葡萄糖 | + | ||
明胶液化 | + | 肌醇 | + | 阿拉伯糖 | + | ||
硝酸盐还原 | + | 果糖 | + | 生长温度范围 | 20-40℃ | ||
石蕊牛奶 | + | 甘露醇 | + | 生长pH范围 | 4-9 | ||
尿素酶 | + | 木糖 | + | 生长耐受NaCl范围 | 2%-8% |
Table 2 Physiological and biochemical characteristics of strain WA11-2-9
检测项目Test item | 结果Result | 检测项目Test item | 结果Result | 检测项目Test item | 结果Result | ||
---|---|---|---|---|---|---|---|
淀粉水解 | + | H2S产生 | + | 葡萄糖 | + | ||
明胶液化 | + | 肌醇 | + | 阿拉伯糖 | + | ||
硝酸盐还原 | + | 果糖 | + | 生长温度范围 | 20-40℃ | ||
石蕊牛奶 | + | 甘露醇 | + | 生长pH范围 | 4-9 | ||
尿素酶 | + | 木糖 | + | 生长耐受NaCl范围 | 2%-8% |
Fig. 6 Molecular biology identification of strain WA11-2-9 A:The 16S rDNA amplified products of strain WA11-2-9. B:The phylogenetic tree of strain WA11-2-9 based on 16S rDNA gene sequence
Fig. 8 HPLC analyzing spectrum of transformed product of strain WA11-2-9 A:The HPLC spectrogram of standard 7-XDT. B:The HPLC spectrogram of standard 10-DAT. C:The HPLC spectrogram of standard 10-DAB. D:The HPLC spectrogram of strain WA11-2-9. E:The HPLC spectrogram of WA11-2-9 contrast group(no 7-XDT added)
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