Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (3): 130-138.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0394

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Isolation and Identification of a Secretory Siderophore Fungus,and Its Biological Activity

ZOU Xue-feng1,2(), LI Ming-gang2, BAO Ling-feng1, CHEN Qi-bin1, ZHAO Jiang-yuan2, WANG Lin3, PU Yong-yu3, HAO Da-cheng4, ZHANG Qing5, YANG Pei-wen5()   

  1. 1. College of Plant Protection,Yunnan Agricultural University,Kunming 650201
    2. Yunnan Institute of Microbiology,Yunnan University,Kunming 650091
    3. Baoshan Tobacco Company of Yunnan Province,Baoshan 678000
    4. School of Environment and Chemical Engineering,Dalian Jiaotong University,Dalian 116028
    5. Institute of Agricultural Environment and Resources,Yunnan Academy of Agricultural Sciences,Kunming 650205
  • Received:2021-03-28 Online:2022-03-26 Published:2022-04-06
  • Contact: YANG Pei-wen E-mail:1054021510@qq.com;pwyang2000@126.com

Abstract:

A secretory siderophore producing fungus was isolated from the soil environment of Ailao Mountain primeval forest in Yunnan National Nature Reserve,and its effects on the physiological and biochemical functions of soil microorganisms infected with tobacco bacterial wilt were studied,which provides a theoretical basis for the development and utilization of the strain. Dilution coating method was used to isolate and purify the fungus producing the secretory siderophore from soil sample,the taxonomic status of the strain was identified by morphology and rDNA-ITS gene sequence. Chromazurol S(CAS)solution detection combined with full band ultraviolet absorption method was to determine the chemical structure type and activity of siderophore. DNS colorimetric method and Biolog-ECO analysis method were used to analyze the effects of strain secondary metabolites on the enzyme activity and microbial metabolism of tobacco bacterial wilt soil. The secretory siderophore-producing fungus was identified as Aspergillus welwitschiae based on morphological observations and sequence alignment analysis;the chemical structure type of the secretory siderophore of the strain was carboxylates,and its siderophore activity was 78.79%. The secondary metabolites of the strain significantly increased the activities of soil invertase and urease(P<0.05)by 13.7% and 14.46% respectively. The secondary metabolites of the strain also significantly improved the carbon source utilization capacity:Average color change rate(AWCD)and diversity index(Shannon index and richness index)increased by 86.15%,50.43% and 66.67% respectively. In conclusion,siderophore microbial resources are stored in primeval forest soil environment. The improvement of physiological and biochemical functions of the soil microbial community mediated by strain-secreted siderophore is an important technical means to optimize the management of the soil ecosystem.

Key words: soil in primeval forest, siderophore, Ralstonia solanaceous, soil enzyme, Biolog-ECO