Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (7): 247-257.doi: 10.13560/j.cnki.biotech.bull.1985.2021-1111

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Isolation of Halophilic Heterotrophic Nitrification-aerobic Denitrification Bacterium Bacillus megatherium N07 and Its Denitrification Characteristics

GAO Yu-xuan1(), JIN Jing-chen2, XU Li-shan1, GAO Ya-Juan1, ZHANG Wen-tian1, LI Chen-chen1, ZHANG Guo-wei1, JIN Yong-sheng1,3()   

  1. 1. School of Biology, Resources and Environment, Beijing Agricultural University, Beijing 102206
    2. Beijing Epoxy Environmental Protection Technology Development Co., Ltd., Beijing 100000
    3. North China Key Laboratory of Urban Agriculture, Ministry of Agriculture and Rural Areas, Beijing 102206
  • Received:2021-08-30 Online:2022-07-26 Published:2022-08-09
  • Contact: JIN Yong-sheng E-mail:1031836790@qq.com;jin6405@126.com

Abstract:

Biological nitrogen-removing technology based on heterotrophic nitrification and aerobic denitrification bacteria may effectively remove nitrogen in sewage. There are many shortcomings in the treatment of high salt wastewater,such as inhibiting microbial growth and low nitrogen removal efficiency. In order to solve the problem of difficult nitrogen removal from high salt wastewater,a strain of heterotrophic nitrification and aerobic denitrification with high salt concentration of 8%(W/V)was selected from chemical wastewater of agricultural pharmaceutical factory and named N07. The denitrification rate of the strain was measured under different salinity and dissolved oxygen conditions,and the effects of single environmental factors on the denitrification performance of the strain was investigated. The results showed that strain N07 was identified as Bacillus megatherium. It tolerated 8% salinity and reproduced vigorously. The removal rates of ammonia nitrogen and nitrite nitrogen were up to 73.61% and 83.56%,belonging to moderate halophilic bacteria. The bacterium showed high denitrification ability under aerobic conditions,with nitrate and nitrite removal rates of 11.87 mg/(L· h)and 8.26 mg/(L· h)at 12 h. The best nitrification capacity was obtained when carbon source was sucrose,C/N was 25,pH was 7,rotation speed was 160 r/min and temperature was 28℃. B. megatherium N07 can degrade nitrogen in high salinity environment,which provides reference for the practical application of heterotrophic nitrification and aerobic denitrification bacteria in the treatment of high salinity nitrogen wastewater such as coastal aquaculture industry.

Key words: heterotrophic nitrification aerobic denitrification, biological nitrogen removal, high salt wastewater, Bacillus megatherium