Research Progress in Anti-porcine Reproductive and Respiratory Syndrome Genetically Modified Pigs

doi:10.13560/j.cnki.biotech.bull.1985.2023-0373

Abstract

Abstract:

Porcine reproductive and respiratory syndrome is a viral infectious disease caused by Porcine reproductive and respiratory syndrome virus（PRRSV）. The disease is one of the three most important diseases affecting the healthy development of pig industry in China. At present, the prevention and control of the disease mainly depends on the internal and external biosecurity system of the farm, taking reasonable measures to reduce the pollution of the virus, and cutting off the circulation and transmission of the virus among pigs. From the perspective of protecting susceptible animals, breeding for disease resistance is also one of the major strategies for disease prevention and control. In recent years, with the development and maturity of gene editing technology, molecular breeding, as the core technology of pig disease resistance breeding, has shown its unique advantages. Several teams have made many breakthroughs in PRRS resistance breeding by using molecular breeding technology. This article takes the PRRSV receptor as the entry point, and reviews the current research status of pig PRRS-resistant breeding, aiming to provide reference and clues for the prevention and control of PRRS and the research of pig disease-resistant breeding.

Key words: porcine reproductive and respiratory syndrome, pig, disease-resistant breeding, CRISPR/Cas9

LI Shuang-xi, HUA Jin-lian. Research Progress in Anti-porcine Reproductive and Respiratory Syndrome Genetically Modified Pigs[J]. Biotechnology Bulletin, 2023, 39(10): 50-57.

Figures/Tables 1

References 52

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编辑位点 Editing site	编辑类型 Editing type	CD163分子表达情况 Expressing situation of CD163	对PRRSV毒株的易感性 Susceptibility to PRRSV strains	体外实验 In vitro	体内实验 In vivo	参考文献 Reference
猪源CD163 分子第7外显子	插入或缺失		对基因2型毒株不易感		猪	[36]
	插入或缺失	未检测到	对基因1型和2型毒株不易感	PAMs	猪	[25]
	人源类CD163蛋白SRCR8结构域编码基因替换猪源CD163分子第7外显子	表达嵌合CD163分子	对基因1型毒株不易感，对基因2型毒株易感	PAMs	猪	[25]
	人源类CD163蛋白SRCR8结构域编码基因替换猪源CD163分子第7外显子	表达嵌合CD163分子	对高致病性毒株不易感		猪	[37]
	插入或缺失	表达缺失SRCR5结构域的CD163	肺泡巨噬细胞（PAMs）和集落刺激因子1刺激的外周血单核细胞（PMMs）对基因I型第1、2和3亚型毒株不易感，PMMs对基因II型毒株不易感	PAMs和PMMs		[38]
		表达缺失SRCR5结构域的CD163	对基因1型第2亚型的高致病性毒株不易感		猪	[37]
		未检测到	对基因2型高致病性毒株不易感		猪	[39]
		表达截短的CD163蛋白（SRCR5结构域缺失41aa，缺失区域包含病毒受体结合位点）	对基因2型毒株JXA1和MY不易感	PAMs	猪	[40]
		未检测到	对基因2型高致病性毒株不易感		猪	[41]

编辑位点 Editing site	编辑类型 Editing type	CD163分子表达情况 Expressing situation of CD163	对PRRSV毒株的易感性 Susceptibility to PRRSV strains	体外实验 In vitro	体内实验 In vivo	参考文献 Reference
猪源CD163 分子第7外显子	插入或缺失		对基因2型毒株不易感		猪	[36]
	插入或缺失	未检测到	对基因1型和2型毒株不易感	PAMs	猪	[25]
	人源类CD163蛋白SRCR8结构域编码基因替换猪源CD163分子第7外显子	表达嵌合CD163分子	对基因1型毒株不易感，对基因2型毒株易感	PAMs	猪	[25]
	人源类CD163蛋白SRCR8结构域编码基因替换猪源CD163分子第7外显子	表达嵌合CD163分子	对高致病性毒株不易感		猪	[37]
	插入或缺失	表达缺失SRCR5结构域的CD163	肺泡巨噬细胞（PAMs）和集落刺激因子1刺激的外周血单核细胞（PMMs）对基因I型第1、2和3亚型毒株不易感，PMMs对基因II型毒株不易感	PAMs和PMMs		[38]
		表达缺失SRCR5结构域的CD163	对基因1型第2亚型的高致病性毒株不易感		猪	[37]
		未检测到	对基因2型高致病性毒株不易感		猪	[39]
		表达截短的CD163蛋白（SRCR5结构域缺失41aa，缺失区域包含病毒受体结合位点）	对基因2型毒株JXA1和MY不易感	PAMs	猪	[40]
		未检测到	对基因2型高致病性毒株不易感		猪	[41]