Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (4): 246-258.doi: 10.13560/j.cnki.biotech.bull.1985.2022-1045

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Identification and Interaction Analysis of SMAS Gene Family in Tea Plant(Camellia sinensis

WANG Yi-qing1,2(), WANG Tao1,2, WEI Chao-ling3, DAI Hao-min1,2, CAO Shi-xian4, SUN Wei-jiang1,2(), ZENG Wen1,2()   

  1. 1. College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002
    2. Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops, Fuzhou 350000
    3. School of Tea and Food Science & Technology, Anhui Agriculture University, Hefei 230061
    4. Wuyi Star Tea Industrial Company Limited, Nanping 354301
  • Received:2022-08-23 Online:2023-04-26 Published:2023-05-16

Abstract:

S-adenosylmethionine synthase(SAMS)is the only enzyme that catalyzes the synthesis of S-adenosylmethionine(SAM)from methionine and ATP. Research shows that SAMS participates in lignin biosynthesis. In this study, we analyzed the expression patterns and protein interaction networks of the SAMS gene family identified from ‘Huangdan’ tea plant(Camellia sinensis)to explore candidate genes of CsSAMS that may be involved in lignin synthesis. Then we identified the members of CsSAMS gene family by bioinformatics based on the genome of ‘Huangdan’, and further analyzed their protein physicochemical properties, phylogenetic tree, chromosome location, gene structure, protein structure and expression pattern. We also studied the protein interaction network by yeast two-hybrid technology(Y2H). Finally, we determined the lignin contents of the first bud and the two leaves of ‘Haungdan’, ‘Tieguanyin’, ‘Jinguanyin’ and ‘Fuding Dahao’ by UV spectrophotometer. The results of bioinformatics analysis showed that 4 CsSAMS gene family members were identified in ‘Huangdan’. The number of encoded amino acids was 345-519, and the isoelectric point ranged from 6.12 to 6.47. The prediction results of subcellular localization revealed that CsSAMS1 was located in the chloroplast, CsSAMS2 and CsSAMS3 were located in the cytoplasm, and CsSAMS4 was located in the cytoskeleton. Expression of CsSAMS and lignin content detection of different tea varieties indicated that CsSAMS2, CsSAMS3 and CsSAMS4 might potentially regulate lignin content. In addition, Y2H results showed that CsSAMS4 formed homodimers with itself. In this study, the physicochemical properties of four CsSAMS members are identified and analyzed, and their functions are predicted. The expression patterns of CsSAMS genes in different tissue sites, under nitrogen and fluoride treatments, as well as the potential involvement of CsSAMS in lignin synthesis process, are clarified.

Key words: Camellia sinensis, SAMS, bioinformatics analysis, lignin, expression analysis, interacting proteins