Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (4): 288-296.doi: 10.13560/j.cnki.biotech.bull.1985.2022-0917

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Comparative Proteomics Analysis of Aeromonas hydrophila Under Enrofloxacin Stress

YAO Jin-dong1,2,3(), TANG Hua-mei1,2,3, YANG Wen-xiao1,2,3, ZHANG Li-shan1,2,3, LIN Xiang-min1,2,3()   

  1. 1. Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, Fujian Agriculture and Forestry University, Fuzhou 350002
    2. Key Laboratory of Crop Ecology and Molecular Physiology(Fujian Agriculture and Forestry University), Fuzhou 350002
    3. School of Life Sciences(Fujian Agriculture and Forestry University), Fuzhou 350002
  • Received:2022-07-24 Online:2023-04-26 Published:2023-05-16

Abstract:

In order to understand the mechanism of bacterial drug resistance under the stress of quinolone antibiotics, this study took Aeromonas hydrophila as the research object, quantitative proteomics was used to compare the protein expression differences in A. hydrophila with and without enrofloxacin treatment. The results showed that total 446 differentially expressed proteins were identified by mass spectrometry, including 233 up-regulated proteins and 213 down-regulated proteins. Bioinformatics analysis showed that A. hydrophila survival may be promoted by up-regulating the expressions of most DNA repair and sulfur metabolism related proteins. Through the growth curve determination, cysteine combined with enrofloxacin can better inhibit the growth of bacteria. Meanwhile, qPCR was used to verify the expression levels of sulfur metabolism-related genes at the mRNA level, and it was uncovered that the differential expression levels of most genes at the transcription level were consistent with the protein level. These results indicate that sulfur metabolism plays an important role in the ENR stress of A. hydrophila.

Key words: Aeromonas hydrophila, enrofloxacin, LC MS/MS, sulfur metabolism, DNA repair