Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (6): 298-307.doi: 10.13560/j.cnki.biotech.bull.1985.2022-1372

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Screening Identification and Degradation Characteristics of Three Iprodione-degrading Strains

PAN Hu1,2,3(), ZHOU Zi-qiong4, TIAN Yun2   

  1. 1. Institute of Agricultural Product Quality Standard and Testing Research, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa 850032
    2. College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128
    3. Agricultural and Livestock Products Engineering Technology Research Center of Tibet Autonomous Region, Lhasa 850032
    4. School of Food Science, Tibet Institute of Agriculture and Animal Husbandry, Nyingchi 860000
  • Received:2022-11-07 Online:2023-06-26 Published:2023-07-07
  • Contact: PAN Hu E-mail:ph2032007@126.com

Abstract:

To isolate the iprodione-degrading microbial resources suitable for Qinghai-Tibet Plateau, three efficient iprodione-degrading strain Y-20, Y-29 and Y-32 were isolated from the greenhouse soil in Tibet by enrichment culture method. These strains were preliminarily identified based on the morphological characteristics, physiological and biochemical characteristics, and 16S rRNA gene sequence analysis. The effects of NaCl concentration(m/V), pH value, temperature, inoculation amount(V/V)and initial iprodione concentration on the iprodione-degradation rates of these strains were also investigated. The metabolic pathways of iprodione and ipaH gene in these strains were analyzed using gas chromatography method and polymerase chain reaction technique, respectively. The results showed that strain Y-20, Y-29 and Y-32 were identified as three species of Microbacterium genus. Under the optimal iprodione-degrading condition of 1.0% NaCl(m/V), pH 7.0, 25-30℃, 5% inoculation amount(V/V)and 100 mg/L initial iprodione concentration, 100 mg/L iprodione was completely degraded by these strains within 8-12 h. These strains decomposed iprodione into N-(3,5-dichlorophenyl)-2,4-dioximidazolidine and isopropyl carbamate, and had a highly similar ipaH gene(99.14%-99.69%). This study provides the strain resources and theoretical basis for the bioremediation of iprodione-contaminated environment at high altitude area.

Key words: iprodione, degradation, enrichment culture, Microbacterium, metabolic pathway, ipaH gene, gas chromatography