In Situ Screening of Carotenoid Degrading Strains and the Application in Improving Quality and Aroma of Cigar

doi:10.13560/j.cnki.biotech.bull.1985.2023-0258

Abstract

Abstract:

Endophyte degrading carotenoids were screened and applied to domestic cigar leaves to increase domestic cigar tobacco leaves（CTLs）aroma and improve sensory quality. CTLs powder was added into in situ medium to simulate the growth environment of tobacco endophytes, and enrichment culture combined with flow cytometry technology was used to screen strains with β-carotene-degrading ability from high-quality cigar tobacco endophytes. The strains inoculated into domestic CTLs, and the contents of carotenoid degradation products were detected and the sensory quality of CTLs was analyzed. Total 21 endophytes had β-carotene degradation rate over 50%, mainly including Agrobacterium, Rhizobium, Sphingobacterium, Enterobacter, etc. These strains made different effects on CTLs sensory quality, among which strains C31, C11 and H4 increased the aroma type, significantly improved the aroma quality, aroma amount, miscellaneous gas, irritation and other CTLs sensory index. The carotenoid-degrading product content from strain C31, C11 and H4 CTLs was 1.38, 1.28 and 1.43 times compared with CK, respectively. The farnesyl acetone, geranyl acetone and megastigmatrienone strengthened the burnt sweet, flowery and woody aroma of CTLs. The three endophytes C31, C11 and H4 obtained by in situ screening can better improve the domestic CTLs sensory quality, and can be further developed and applied as microbial preparations of new cigar industry and characteristic aromatic cigar.

Key words: carotenoids, degradation, endophyte, situ screening, cigar tobacco leaves, fermentation, quality improvement, aroma enhancement

WU Qiao-yin, SHI You-zhi, LI Lin-lin, PENG Zheng, TAN Zai-yu, LIU Li-ping, ZHANG Juan, PAN Yong. In Situ Screening of Carotenoid Degrading Strains and the Application in Improving Quality and Aroma of Cigar[J]. Biotechnology Bulletin, 2023, 39(9): 192-201.

Figures/Tables 7

References 32

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菌株 Strain	香气质 Aroma quality	香气量 Aroma amount	杂气 Miscellaneous gas	刺激性Thrillness	余味 Remaining taste	甜润感Sweetness	燃烧性Flammability	灰色Gray	质量得分 Quality score
CK	3.10	3.10	2.90	2.90	3.10	2.90	4.10	4.00	60.47
C31	3.50	3.30	3.00	3.30	3.30	3.50	4.50	4.00	67.07
C11	3.30	3.50	3.30	3.50	3.30	3.30	4.50	3.50	66.93
H4	3.00	3.00	3.00	3.30	3.30	2.80	4.00	3.80	63.00
A72	3.30	3.30	3.50	3.30	3.00	2.80	4.50	4.00	62.73
F5	3.00	3.30	3.00	2.80	3.30	2.80	4.00	3.50	61.07
1D3	3.50	3.00	3.50	3.00	3.00	2.50	4.00	4.00	61.00
A5	3.00	2.80	2.80	3.00	2.50	2.30	4.00	3.00	60.93
H92	2.90	3.10	3.10	3.20	2.70	2.80	3.80	3.70	60.83
2F2	3.00	3.00	3.00	3.50	3.00	2.50	3.80	3.50	59.87
2B1	2.80	2.80	2.80	3.20	3.10	2.80	3.30	3.20	59.75
C7	3.30	3.30	3.00	2.50	3.30	2.80	3.00	3.50	59.73
A71	3.30	2.50	3.00	2.50	2.80	2.80	4.00	4.00	59.73
F92	3.00	2.80	3.00	2.80	2.80	2.50	4.00	3.50	59.73
2B2	2.50	2.80	2.80	2.80	2.80	3.00	4.00	4.00	59.33
1F9	2.50	2.50	2.50	2.50	2.50	2.50	4.00	4.00	58.80
2E5	3.00	2.50	3.00	3.00	3.00	3.50	4.00	4.00	57.00
2C11	3.00	3.50	3.00	3.00	3.00	2.50	3.00	3.00	57.30
1C1	2.50	2.50	3.00	3.00	3.00	2.50	4.00	3.00	56.70
B11	2.80	2.80	2.50	3.00	2.80	1.50	4.00	3.30	56.00
F91	3.00	2.80	2.50	2.50	3.00	1.50	3.50	3.00	55.47
C42	3.00	2.80	2.50	2.80	2.80	2.80	3.30	3.30	55.07

菌株 Strain	香气质 Aroma quality	香气量 Aroma amount	杂气 Miscellaneous gas	刺激性Thrillness	余味 Remaining taste	甜润感Sweetness	燃烧性Flammability	灰色Gray	质量得分 Quality score
CK	3.10	3.10	2.90	2.90	3.10	2.90	4.10	4.00	60.47
C31	3.50	3.30	3.00	3.30	3.30	3.50	4.50	4.00	67.07
C11	3.30	3.50	3.30	3.50	3.30	3.30	4.50	3.50	66.93
H4	3.00	3.00	3.00	3.30	3.30	2.80	4.00	3.80	63.00
A72	3.30	3.30	3.50	3.30	3.00	2.80	4.50	4.00	62.73
F5	3.00	3.30	3.00	2.80	3.30	2.80	4.00	3.50	61.07
1D3	3.50	3.00	3.50	3.00	3.00	2.50	4.00	4.00	61.00
A5	3.00	2.80	2.80	3.00	2.50	2.30	4.00	3.00	60.93
H92	2.90	3.10	3.10	3.20	2.70	2.80	3.80	3.70	60.83
2F2	3.00	3.00	3.00	3.50	3.00	2.50	3.80	3.50	59.87
2B1	2.80	2.80	2.80	3.20	3.10	2.80	3.30	3.20	59.75
C7	3.30	3.30	3.00	2.50	3.30	2.80	3.00	3.50	59.73
A71	3.30	2.50	3.00	2.50	2.80	2.80	4.00	4.00	59.73
F92	3.00	2.80	3.00	2.80	2.80	2.50	4.00	3.50	59.73
2B2	2.50	2.80	2.80	2.80	2.80	3.00	4.00	4.00	59.33
1F9	2.50	2.50	2.50	2.50	2.50	2.50	4.00	4.00	58.80
2E5	3.00	2.50	3.00	3.00	3.00	3.50	4.00	4.00	57.00
2C11	3.00	3.50	3.00	3.00	3.00	2.50	3.00	3.00	57.30
1C1	2.50	2.50	3.00	3.00	3.00	2.50	4.00	3.00	56.70
B11	2.80	2.80	2.50	3.00	2.80	1.50	4.00	3.30	56.00
F91	3.00	2.80	2.50	2.50	3.00	1.50	3.50	3.00	55.47
C42	3.00	2.80	2.50	2.80	2.80	2.80	3.30	3.30	55.07

类胡萝卜素降解产物含量Content of carotenoid degradation product/（μg·g^-1）	对照CK	C11	C31	H4
柑橘酮3,7-Nonadien-2-one, 4,8-dimethyl	-	-	-	0.05
香叶基丙酮Geranyl acetone	0.60	1.04	0.97	0.96
氧化紫罗兰醇（+）-3-Oxo-alpha-ionol	-	0.14	-	0.08
巨豆三烯酮Megastigmatrienone	0.70	0.91	0.27	0.50
橙化基丙酮Neryl acetone	0.60	-	0.79	0.96
六氢假紫罗兰酮2-Undecanone, 6,10-dimethyl	0.06	0.09	0.13	0.09
二氢猕猴桃内酯Dihydroactinidiolide	0.45	0.66	0.64	0.53
4-氧化异佛尔酮4-Oxoisophorone	0.01	0.03	-	0.04
法尼基丙酮Farnesyl acetone	0.38	0.83	0.98	0.68
甲基庚烯酮5-Hepten-2-one, 6-methyl	0.03	0.09	0.18	0.19
β-二氢紫罗兰酮Dihydro-beta-ionone	0.06	-	-	0.05
总量Total	2.88	3.70	3.96	4.13

类胡萝卜素降解产物含量Content of carotenoid degradation product/（μg·g^-1）	对照CK	C11	C31	H4
柑橘酮3,7-Nonadien-2-one, 4,8-dimethyl	-	-	-	0.05
香叶基丙酮Geranyl acetone	0.60	1.04	0.97	0.96
氧化紫罗兰醇（+）-3-Oxo-alpha-ionol	-	0.14	-	0.08
巨豆三烯酮Megastigmatrienone	0.70	0.91	0.27	0.50
橙化基丙酮Neryl acetone	0.60	-	0.79	0.96
六氢假紫罗兰酮2-Undecanone, 6,10-dimethyl	0.06	0.09	0.13	0.09
二氢猕猴桃内酯Dihydroactinidiolide	0.45	0.66	0.64	0.53
4-氧化异佛尔酮4-Oxoisophorone	0.01	0.03	-	0.04
法尼基丙酮Farnesyl acetone	0.38	0.83	0.98	0.68
甲基庚烯酮5-Hepten-2-one, 6-methyl	0.03	0.09	0.18	0.19
β-二氢紫罗兰酮Dihydro-beta-ionone	0.06	-	-	0.05
总量Total	2.88	3.70	3.96	4.13