Biotechnology Bulletin ›› 2024, Vol. 40 ›› Issue (7): 207-215.doi: 10.13560/j.cnki.biotech.bull.1985.2024-0152
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REN Xiao-min1(), YUN Lan1,2(), AI Qian1, ZHAO Qiao3()
Received:
2024-02-11
Online:
2024-07-26
Published:
2024-05-24
Contact:
YUN Lan, ZHAO Qiao
E-mail:2937923090@qq.com;yunlan@imau.edu.cn;qiao.zhao@siat.ac.cn
REN Xiao-min, YUN Lan, AI Qian, ZHAO Qiao. Functional Verification of Isopentenyl Transferases PjIPT Gene in Psathyrostachys juncea[J]. Biotechnology Bulletin, 2024, 40(7): 207-215.
名称 Name | 正向引物 Forward primer(5'-3') | 反向引物 Reverse primer(5'-3') |
---|---|---|
RT-qPCR | GCAAGCCATGGAGTACCTGT | TCTCATTGCGGAACCAGGTC |
Actin | AGTGGTCGTACAACCGGTATTGT | GAGGAAGAGCATACCCCTCGTA |
RT-IPT | CTTTCGTGAGAGTGGGCAGT | GAAGCGTCAACCCACTGGTA |
Table 1 List of primers
名称 Name | 正向引物 Forward primer(5'-3') | 反向引物 Reverse primer(5'-3') |
---|---|---|
RT-qPCR | GCAAGCCATGGAGTACCTGT | TCTCATTGCGGAACCAGGTC |
Actin | AGTGGTCGTACAACCGGTATTGT | GAGGAAGAGCATACCCCTCGTA |
RT-IPT | CTTTCGTGAGAGTGGGCAGT | GAAGCGTCAACCCACTGGTA |
Fig. 2 Multiple sequence alignment and phylogenetic analysis based on IPT amino acid sequence A: Multiple sequence alignment of IPT proteins in different species. B: Phylogenetic tree of IPT in different species. Os: Oryza sativa; At: Arabidopsis thaliana
Fig. 5 Phenotypic analysis of 35S:p1300-cYFP-PjIPT positive plants A: Axillary branching of wild-type and overexpressed PjIPT in the representative plants described in B. B: Branch numbers and plant height in 42-day-old Col-0 and 3 independent 35S:p1300-cYFP-PjIPT transgenic Arabidopsis lines. ***: Extremely significant difference in(P <0.001). The same below
Fig. 6 Quantitative analysis of trans-zeatin A: Analysis of trans-zeatin content in different samples; B: MRM chromatograms; C: MRM mass spectrographs
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