Biotechnology Bulletin ›› 2024, Vol. 40 ›› Issue (7): 323-334.doi: 10.13560/j.cnki.biotech.bull.1985.2024-0076

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Whole Genome Sequencing and Bioinformatics Analysis of Mycoplasma ovipneumoniae GH3-3 Strain

TIAN Tong-tong1,2(), GE Jia-zhen1, GAO Peng-cheng1, LI Xue-rui1, SONG Guo-dong1, ZHENG Fu-ying1(), CHU Yue-feng1,2()   

  1. 1. State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730000
    2. College of Veterinary Medicine,Xinjiang Agricultural University,Urumqi 830052
  • Received:2024-01-17 Online:2024-07-26 Published:2024-07-30
  • Contact: ZHENG Fu-ying, CHU Yue-feng E-mail:t1123859519@163.com;zhengfuying@caas.cn;chuyuefeng@caas.cn

Abstract:

【Objective】 This study is aimed to decode the genomic sequence of Mycoplasma ovipneumoniae strain GH3-3, to investigate its pathogenic potential and to elucidate the regulatory mechanisms of its replication, transcription, and translation. 【Method】 M. ovipneumoniae GH3-3 strain was cultured in vitro. When it reached the late logarithmic stage of growth, the genomic DNA of M. ovipneumoniae GH3-3 strain was extracted with bacterial genomic DNA extraction kit and sent for whole genome sequencing and commentary. 【Result】 The genome size of GH3-3 strains of M. ovipneumoniae was 1 060 772 bp, and the GC content was 29.66%. The genome component analysis showed that the genome of GH3-3 strain contained 730 coding genes, with a total length of 914 379 bp and an average length of 1 252.57 bp, accounting for 86.2% of the total genome length. There were 149 tandem repeats with a total length of 20 926 bp, accounting for 1.97% of the total length of the genome. There were 102 microsatellite DNA sequences, 30 tRNA sequences, and 3 rRNA sequences. The 719, 459, 473, 394, 449, 33, 5, 180, 113 and 59 genes were annotated in NR, SwissProt, GOG, KEGG, GO, CARD, CAZy, PHI, TCDB, and RMS databases, respectively. A total of 76 virulence factor-related genes were annotated. The genome sequence was submitted to the NCBI website with the login number: PRJNA1051969. 【Conclusion】 The complete genome information of M. ovipneumoniae GH3-3 strain was acquired, and the gene function was predicted and annotated. Furthermore, the genetic and evolutionary relationships between GH3-3 strain and other strains of M. ovipneumoniae were elucidated globally.

Key words: Mycoplasma ovipneumoniae, GH3-3 strain, whole genome sequencing(WGS), virulence factor, drug-resistant gene