Differential Expression Analysis of Genes Related to NaCl Stress Response in Lycium barbarum 'Ningqi 1'

doi:10.13560/j.cnki.biotech.bull.1985.2024-0632

Abstract

Abstract:

Objective To explore the differential expression patterns of signal transduction pathways-related genes in Lycium barbarum Ningqi 1 under salt stress, and to lay a foundation for a deep understanding of the molecular mechanisms of L. barbarum 'Ningqi 1' tolerance to salinity. Method Transcriptome sequencing technique was used to analyze the signal transduction pathways-related differentially expressed genes in the leaves of L.barbarum Ningqi 1 under different concentrations of NaCl stress, and the changes in enzyme activities related to these pathways were also examined. Result 1) A total of 14 genes related to three signal transduction pathways were differentially expressed in the leaves of L.barbarum Ningqi 1 after 7 d of treatment with 0, 100, 200 and 300 mmol/L NaCl stress. 2) The relative expression of CIPK6 showed a downward trend, while that of MAPKK2 was in an upward trend. The relative expressions of MAPKKK18 and MAPK3 initially increased and then decreased with NaCl concentration increased. The RT-qPCR results were basically consistent with those obtained from RNA-seq. 3) As the NaCl concentration increasing, the enzyme activities of CIPK6, MAPKK2 and MAPKKK18 initially rose and then unchanged. The enzyme activities of MAPK3 and PLD were significantly higher compared to the control group. The content of calmodulin increased with the rising NaCl concentration. Conclusion L.barbarum Ningqi 1 may respond to NaCl stress by inducing phosphatidylinositol, MAPK cascade and differential expression of Ca²⁺dependent SOS signal transduction-related genes, and thus improve its tolerance to salt.

Key words: Lycium barbarum 'Ningqi 1', NaCl stress, signal transduction, differential expression of genes

LI Jing-jing, HU Jin-hong, LIANG Wang-li, MA Yu-rong, LIANG Wen-yu, WANG Ling-xia. Differential Expression Analysis of Genes Related to NaCl Stress Response in Lycium barbarum 'Ningqi 1'[J]. Biotechnology Bulletin, 2025, 41(2): 202-209.

Figures/Tables 7

References 39

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基因名称 Gene name	引物名称 Primer name	序列 Sequence （5′‒3′）
actin	actin-F	GGTCCTCTTCCAGCCATCCAT
actin	actin-R	TGAGCCACCACTGAGCACAA
CIPK6	CIPK6-F	CTTTCTCCCACACTCTTGCCCTTG
CIPK6	CIPK6-R	GCTTGAGGAGGTGGCGAAAACG
MAPKKK18	MAPKKK18-F	CGTATGTGTAGCTGTCACCAAGGC
MAPKKK18	MAPKKK18-R	GTCTTGTTCCGCTTCCTGCTCAG
MAPKK2	MAPKK2-F	GGCACCTCCTGAAATTCTCTCTGG
MAPKK2	MAPKK2-R	CTGGCAAGCACATCTGGACTGG
MAPK3	MAPK3-F	GGCACCCCAACGGAATCTGATC
MAPK3	MAPK3-R	CTGCTAACTGCTGGCGTGGATG

基因名称 Gene name	引物名称 Primer name	序列 Sequence （5′‒3′）
actin	actin-F	GGTCCTCTTCCAGCCATCCAT
actin	actin-R	TGAGCCACCACTGAGCACAA
CIPK6	CIPK6-F	CTTTCTCCCACACTCTTGCCCTTG
CIPK6	CIPK6-R	GCTTGAGGAGGTGGCGAAAACG
MAPKKK18	MAPKKK18-F	CGTATGTGTAGCTGTCACCAAGGC
MAPKKK18	MAPKKK18-R	GTCTTGTTCCGCTTCCTGCTCAG
MAPKK2	MAPKK2-F	GGCACCTCCTGAAATTCTCTCTGG
MAPKK2	MAPKK2-R	CTGGCAAGCACATCTGGACTGG
MAPK3	MAPK3-F	GGCACCCCAACGGAATCTGATC
MAPK3	MAPK3-R	CTGCTAACTGCTGGCGTGGATG

基因名称 Gene name	B vs A		C vs A		D vs A		基因注释 Gene description
基因名称 Gene name	差异倍数 Log₂Fold Change	P值 P value	差异倍数 Log₂Fold Change	P值 P value	差异倍数 Log₂Fold Change	P值 P value	基因注释 Gene description
MAPK3	1.78	0	-1.12	6.18E-108	-1.61	1.84E-232	有丝分裂原活化蛋白激酶3 Mitogen-activated protein kinase 3
MAPK4	1.48	2.88E-07	1.53	1.34E-09	-	-	有丝分裂原活化蛋白激酶4 Mitogen-activated protein kinase 4
MAPKK2	1.17	2.04E-224	-	-	-	-	有丝分裂原活化蛋白激酶激酶2 Mitogen-activated protein kinase kinase 2
MAPKKK18	4.88	0	-3.63	2.30E-29	-3.027 4	2.84E-32	有丝分裂原活化蛋白激酶激酶激酶18 Mitogen-activated protein kinase kinase kinase 18
GPAT1	1.51	1.45E-75	-0.27	0	0.67	5.03E-18	甘油-3-磷酸酰基转移酶1 Glycerol-3-phosphate acyltransferase 1
GPAT6	1.05	5.12E-109	-	-	-	-	甘油-3-磷酸 2-O-酰基转移酶6 Glycerol-3-phosphate 2-O-acyltransferase 6
PLDα1	1.85	0	1.53	8.54E-253	1.06	1.71E-106	磷脂酶D α1 Phospholipase D α1
DGK1	2.89	2.25E-191	1.95	1.22E-76	1.04	6.46E-22	二酰基甘油激酶1 Diacylglycerol kinase 1
CBL4	1.56	1.67E-19	-1.13	3.77E-07	3.28	9.49E-248	钙调磷酸酶B样蛋白4 Calcineurin B-like protein 4
CaM3	-	-	2.01	1.93E-25	-	-	钙调蛋白-3 Calmodulin-3
CaM2	-	-	3.12	0	-	-	钙调蛋白-2 Calmodulin-2
CaM	0.64	5.68E-44	1.06	1.53E-170	1.06	4.64E-235	钙调蛋白 Calmodulin
CIPK9	-0.34	9.32E-31	-	-	-	-	CBL相互作用的丝氨酸/苏氨酸蛋白激酶9 CBL-interacting serine/threonine-protein kinase 9
CIPK6	-0.94	2.14E-15	-	-	-1.48	7.62E-44	CBL相互作用的丝氨酸/苏氨酸蛋白激酶6 CBL-interacting serine/threonine-protein kinase 6

基因名称 Gene name	B vs A		C vs A		D vs A		基因注释 Gene description
基因名称 Gene name	差异倍数 Log₂Fold Change	P值 P value	差异倍数 Log₂Fold Change	P值 P value	差异倍数 Log₂Fold Change	P值 P value	基因注释 Gene description
MAPK3	1.78	0	-1.12	6.18E-108	-1.61	1.84E-232	有丝分裂原活化蛋白激酶3 Mitogen-activated protein kinase 3
MAPK4	1.48	2.88E-07	1.53	1.34E-09	-	-	有丝分裂原活化蛋白激酶4 Mitogen-activated protein kinase 4
MAPKK2	1.17	2.04E-224	-	-	-	-	有丝分裂原活化蛋白激酶激酶2 Mitogen-activated protein kinase kinase 2
MAPKKK18	4.88	0	-3.63	2.30E-29	-3.027 4	2.84E-32	有丝分裂原活化蛋白激酶激酶激酶18 Mitogen-activated protein kinase kinase kinase 18
GPAT1	1.51	1.45E-75	-0.27	0	0.67	5.03E-18	甘油-3-磷酸酰基转移酶1 Glycerol-3-phosphate acyltransferase 1
GPAT6	1.05	5.12E-109	-	-	-	-	甘油-3-磷酸 2-O-酰基转移酶6 Glycerol-3-phosphate 2-O-acyltransferase 6
PLDα1	1.85	0	1.53	8.54E-253	1.06	1.71E-106	磷脂酶D α1 Phospholipase D α1
DGK1	2.89	2.25E-191	1.95	1.22E-76	1.04	6.46E-22	二酰基甘油激酶1 Diacylglycerol kinase 1
CBL4	1.56	1.67E-19	-1.13	3.77E-07	3.28	9.49E-248	钙调磷酸酶B样蛋白4 Calcineurin B-like protein 4
CaM3	-	-	2.01	1.93E-25	-	-	钙调蛋白-3 Calmodulin-3
CaM2	-	-	3.12	0	-	-	钙调蛋白-2 Calmodulin-2
CaM	0.64	5.68E-44	1.06	1.53E-170	1.06	4.64E-235	钙调蛋白 Calmodulin
CIPK9	-0.34	9.32E-31	-	-	-	-	CBL相互作用的丝氨酸/苏氨酸蛋白激酶9 CBL-interacting serine/threonine-protein kinase 9
CIPK6	-0.94	2.14E-15	-	-	-1.48	7.62E-44	CBL相互作用的丝氨酸/苏氨酸蛋白激酶6 CBL-interacting serine/threonine-protein kinase 6

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