Biotechnology Bulletin ›› 2013, Vol. 0 ›› Issue (10): 28-33.

• Review • Previous Articles     Next Articles

Application of SUMO Protein in Fusion Expression System

Wang Xiaojie, Mao Ruoyu, Zhang Yong, Teng Da, Wang Xiumin, Wang Jianhua   

  1. （Key Laboratory of Feed Biotechnology of MOA，Gene Engineering Laboratory，Feed Research Institute，Chinese Academy of AgriculturalSciences，Beijing 100081）
  • Received:2013-04-17 Revised:2013-10-15 Online:2013-10-14 Published:2013-10-15

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Abstract:

The expression of toxic protein is more challenging than common protein. Fusion expression strategy may be a good choice to reslove this problem. Traditional fusion tags including thioredoxin, green fluorescent protein, maltose binding protein, ketosteroid isomerase, NusA, glutathione-s-transferase have been widely used. Besides the characteristics of the traditional fusion tags above, SUMO protein has other advantages, such as small molecules, promoting folding and specific cleaved by SUMO protease 1. As a result, it has been widely used recently.
This article depicted the characteristics of SUMO and SUMO pretease. Additonally, the application of SUMO fusion expression system was elaborated.

Key words: SUMO（smallubiquitin-related modifier）, Fusion expression, SUMO protease Ⅰ, Toxic protein