Biotechnology Bulletin ›› 2020, Vol. 36 ›› Issue (9): 235-243.doi: 10.13560/j.cnki.biotech.bull.1985.2020-0079

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Fusion Expression of Somatostatin with a Thermostable Xylanase and Characterization of the Fusion Protein

HUANG Kun-long, SU Xiao-yun, YAO Bin   

  1. Key Laboratory of Feed Biotechnology of Ministry of Agriculture,Feed Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081
  • Received:2020-01-02 Online:2020-09-26 Published:2020-09-30

Abstract: The aim of this study is to obtain the fusion protein CbXyn10C-SS with antigenicity and immunogenicity,to efficiently express it,and to characterize the enzymatic properties. Somatostatin(SS)impedes feed transformation efficiency and animal growth in multiple ways. Therefore,using SS to immunize animals can avoid the inhibitory effect. However,the research of SS immunization mostly uses DNA vaccine as the carrier and highly efficient recombinant production of SS protein is seldom reported. Xylanase is widely used in feed,which can improve feed conversion rate. Till now,recombinant xylanase has been successfully expressed in microbial cells. The SS gene was fused to the 3' terminus of the thermostable xylanase gene CbXyn10C derived from the Caldicellulosiruptor bescii. The fusion protein CbXyn10C-SS presented antigenicity and immunogenicity. Being highly thermostable and efficient in catalysis,CbXyn10C-SS also demonstrated very similar biochemical characteristics and catalytic parameters to those of CbXyn10C,which is therefore suitable for the feed pelleting process. The successfully and efficiently expressed fusion protein CbXyn10C-SS has potential application value as a feed xylanase and somatostatin antigen.

Key words: somatostatin, xylanase, fusion expression, antigenic epitope