Biotechnology Bulletin ›› 2013, Vol. 0 ›› Issue (10): 66-70.

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Specific Detection of GM Maize Events Using Aldehyde PCR Chip

Zhang Mingzhe1,Zhang Xiaofeng1,Chen Xi1,Wu Shan1,Chen Xiaomei1,Zhou Yuan2,   

  1. （1. Zhejiang Academy of Science and Technology for Inspection and Quarantine，Hangzhou 310016 ；2. Zhoushan Entry-exit Inspection and Quarantine Bureau，Zhoushan 316000）
  • Received:2013-07-17 Revised:2013-10-15 Online:2013-10-14 Published:2013-10-15

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Abstract:

In this study, 9 kinds of genetically modified maize events Bt11, TC1507, Bt176, MON810, MON863, GA21, NK603, Mon88017, MIR604 were under high-throughput detection and condition optimization with aldehyde PCR chips. According to the specificity and sensitivity experiments, it showed that the specificity of the aldehyde PCR chip is good enough to be applied in the identification of genetically modified maize events. But the sensitivity of this method was 5%, if it is applied to the transgenic detection. It calls for further optimization in the follow-up experiments.

Key words: Aldehyde PCR chip, Foreign gene detection, Identification of genetic modified events