Abstract:

The paper described a simple system to product ha FGF protein in pea plants by seed absorbing the Agrobacterium suspension containing PEBV-based vectors, and the expression results was detected by semi-quantitative RT-PCR, Western blotting, and Coomassie brilliant blue method. It has been shown that GFP and ha FGF protein were successfully expressed in pea（Pisum sativum L.）plants. The optimization of Agrobacterium cell density dramatically improved expression efficiency of GFP protein. The amount of recombinant pharmaceutical ha FGF in plants reached up to 2.6% of the total soluble proteins. The new system has the special advantages as easiest way, high efficiency, stable expression, and has the wide application in production of pharmaceutical proteins in the future.

Key words: Human acidic fibroblast growth factor（ha FGF）, Seed absorption system, Pea early browning virus（PEBV）, GFP