Biotechnology Bulletin ›› 2013, Vol. 0 ›› Issue (10): 76-80.

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Cloning and Sequence Analysis of 1-aminocyclopropane-1-carboxylate （ACC）Oxidase Gene cDNA from Gynoecious Momordica charantia

Wang Risheng1,2,, Zhang Man1,Huang Rukui1,Liu Wenjun1,Dong Wenbin1,Che Jianglü2, Fang Fengxue1,Li Yangrui2,   

  1. （1. Vegetable Research Institute，Guangxi Academy of Agricultural Sciences，Nanning 530007 ；2. Guangxi Crop Genetic Improvement and Biotechnology Lab，Nanning 530007）
  • Received:2013-04-01 Revised:2013-10-15 Online:2013-10-14 Published:2013-10-15

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Abstract:

The full cDNA sequence of 1-aminocyclopropane-1-carboxylic acid oxidase（ACO）gene was cloned by RT-PCR and RACE from floral buds of gynoecious bitter melon‘X-Hei-d-d’, which was named as Mc-ACO1（GenBank accession No. FJ459813）. The full length cDNA of Mc-ACO1 is 1 137 bp, which includes 1 005 bp complete CDS and encodes 334 amino acid residues with a putative molecular mass of 37.30 kD. The protein encoded by Mc-ACO1 gene lacks one typically conserved domain and two amino acid residues of Fe2+ ascorbate family of dioxygenases. The bioinformatics characterization indicate that the evolution of plant ACO has higher consistence with their genetic relationship ； and the different amino acid sequences of Mc-ACO1 are mainly at the N-terminal area compared with those of other plants ；Mc-ACO1 should belong to the same member as cucumber Cs-ACO2, and the presumed function of Mc-ACO1 is probably related with sex differentiation.

Key words: Gynoecious bitter melon, Sex differentiation, ACC oxidase, Gene clone, Sequence analysis