Biotechnology Bulletin ›› 2013, Vol. 0 ›› Issue (10): 131-136.

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Colonization of Bacillus subtilis BS24 on the Apple Leaf Surface and Their Effects on the Leaf Microbial Flora

Ran Ganqiao1,Wang Nan2, Dai Jiakun3, Zhao Wenjuan1,Ren Ping1,Qin Tao1,   

  1. （1. Institute of Enzyme Engineering，Shaanxi Academy of Science，Xi’an 710600 ；2. School of Food and Bioengineering，Zhengzhou University of Light Industry，Zhengzhou 450001 ；3. Enzyme Engineering Technology Center of Shaanxi Province，Xi’an 710600）
  • Received:2013-05-14 Revised:2013-10-15 Online:2013-10-14 Published:2013-10-15

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Abstract:

To confirm the biocontrol efficacy of the Bacillus subtilis BS24 strain against apple early defoliation disease, the colonization of BS24 strains on apple leaves and their effects on the other microbial species and populations were studied respectively. The results showed that under field conditions BS24 strains could colonize on apple leaves, but the population size declined with prolonging time. In the absence of rain and other inclement weather situations, the population size of BS24 strain increased from the initial 2.5×107 cfu/g to 2.1×105cfu/g after spraying the microbial agents for 15 days. Compared with the apple leaf microorganisms without spraying agents, the microbial populations on apple leaves declined after 20 days of spraying agents, meanwhile the bacteria of Pseudomonas oryzihabitans, Acinetobacter lwoffii, Bacillus lehensis and Kocuria rosea and the fungi of the Alternaria alternate, Eupenicillium janthinellum and Verticillium dahliae were not detected.

Key words: Apple early defoliation diseases, Bacillus subtilis, Colonization, Isolation and purification of microorganism, Molecular identification