Transcription of Ethylene Biosynthesis and Signaling Associated Genes in Response to Heterodera glycine Infection

doi:10.13560/j.cnki.biotech.bull.1985.2021-1414

Abstract

Abstract:

Ethylene is an important plant hormone. Its synthesis and signal transduction related genes are involved in the regulation of plant growth,development and defense response. In order to clarify the effects of soybean cyst nematode infection and colonization on ethylene biosynthesis and signal transduction pathway,the expressions of ethylene synthesis gene GmACS and signal transduction gene GmEIN in soybean roots inoculated with and without cyst nematode were compared by fluorescence quantitative PCR. The results showed that compared with susceptible varieties,the ethylene biosynthesis in resistant varieties was significantly regulated,and in which 6 GmACSs genes were significantly induced in PI 548316,while significantly inhibited in PI 437654 and PI 88788. Meanwhile,several GmEINs was slightly up-regulated in 4 resistant varieties,but not significantly regulated in other resistant varieties and two susceptible varieties. The results show that ethylene synthesis in some soybean varieties are significantly regulated by soybean cyst nematode infection and colonization,and the regulated genes can be used as candidates for improving soybean disease resistance breeding.

Key words: soybean, Heterodera glycines, GmACS, GmEIN, gene expression pattern

GUO Bin-hui, SONG Li. Transcription of Ethylene Biosynthesis and Signaling Associated Genes in Response to Heterodera glycine Infection[J]. Biotechnology Bulletin, 2022, 38(8): 150-158.

Figures/Tables 6

References 29

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基因名称Gene name	基因 ID Gene ID	正向引物Forward primer	反向引物Reverse primer
GmACS#009	Glyma.07G065700	ATGTGAGCGTTGCTGAAGTG	AAGCCAGGGTACCCCATATC
GmACS#018	Glyma.16G032200	TAAGGACATGGGGTTTCCTG	TCCGAAACTCGACATTTTCC
GmACS#014	Glyma.09G255000	CTTGAAGGGTTTGCTGAAGG	CTGACCCTGGTGACACATTG
GmACS#021	Glyma.18G238100	TCATGGATTGCCAGAGTTCA	CCCCACTCATCAAAATACGG
GmACS#007	Glyma.05G211700	GGGTGGTTTAGGGTGTGCTA	GGCACCATGATCTCCTTGTT
GmACS#011	Glyma.08G018000	CTGGCTCTTCTTTCCATTGC	CGAAGGTTCGAATTCTTTGC
GmACS#015	Glyma.11G021500	GCGACGAAATATACGCTGGT	ATAAGGTCGCGGTCACATTC
GmACS#005	Glyma.05G108900	CGTATCGGGGCTATTGAAAA	GAACGTGTTCGAATGCAAAA
GmACS#020	Glyma.17G158100	TACCTCCTCTCTGCCATGCT	CCGAAACGAGCATTTTCTGT
GmEIN#001	Glyma.02G274600	CAAAGCCCTCCACCTTACAA	TTGGCAATATCAGGGGACAT
GmEIN#005	Glyma.13G076800	TTGGGATGGAAAGAGTGAGG	CCTCTTCCGAATGAAATCCA
GmEIN#006	Glyma.13G076700	CAAAATTTGCAAGCTCGTGA	TGCCAAGTCCTCCTCTTGAT
GmEIN#008	Glyma.14G041500	TATGATGTTGATGGGGCTGA	CATTCTCTCCATCCCAAGG
GmEIN#010	Glyma.20G051500	CAGAGAAGGGAAAGCCAGTG	GTTATGGCAGCAGGACCATT
GmEIN#007	Glyma.13G145100	GGCATCATTGATCTGGCTTT	GGAGGTGAGCCTGACTTCTG
GmACT11	Glyma.18G290800	ATCTTGACTGAGCGTGGTTATTCC	GCTGGTCCTGGCTGTCTCC

基因名称Gene name	基因 ID Gene ID	正向引物Forward primer	反向引物Reverse primer
GmACS#009	Glyma.07G065700	ATGTGAGCGTTGCTGAAGTG	AAGCCAGGGTACCCCATATC
GmACS#018	Glyma.16G032200	TAAGGACATGGGGTTTCCTG	TCCGAAACTCGACATTTTCC
GmACS#014	Glyma.09G255000	CTTGAAGGGTTTGCTGAAGG	CTGACCCTGGTGACACATTG
GmACS#021	Glyma.18G238100	TCATGGATTGCCAGAGTTCA	CCCCACTCATCAAAATACGG
GmACS#007	Glyma.05G211700	GGGTGGTTTAGGGTGTGCTA	GGCACCATGATCTCCTTGTT
GmACS#011	Glyma.08G018000	CTGGCTCTTCTTTCCATTGC	CGAAGGTTCGAATTCTTTGC
GmACS#015	Glyma.11G021500	GCGACGAAATATACGCTGGT	ATAAGGTCGCGGTCACATTC
GmACS#005	Glyma.05G108900	CGTATCGGGGCTATTGAAAA	GAACGTGTTCGAATGCAAAA
GmACS#020	Glyma.17G158100	TACCTCCTCTCTGCCATGCT	CCGAAACGAGCATTTTCTGT
GmEIN#001	Glyma.02G274600	CAAAGCCCTCCACCTTACAA	TTGGCAATATCAGGGGACAT
GmEIN#005	Glyma.13G076800	TTGGGATGGAAAGAGTGAGG	CCTCTTCCGAATGAAATCCA
GmEIN#006	Glyma.13G076700	CAAAATTTGCAAGCTCGTGA	TGCCAAGTCCTCCTCTTGAT
GmEIN#008	Glyma.14G041500	TATGATGTTGATGGGGCTGA	CATTCTCTCCATCCCAAGG
GmEIN#010	Glyma.20G051500	CAGAGAAGGGAAAGCCAGTG	GTTATGGCAGCAGGACCATT
GmEIN#007	Glyma.13G145100	GGCATCATTGATCTGGCTTT	GGAGGTGAGCCTGACTTCTG
GmACT11	Glyma.18G290800	ATCTTGACTGAGCGTGGTTATTCC	GCTGGTCCTGGCTGTCTCC