Isolation and Identification of Endophytic Bacteria in Leaf from Xiangshan Smoke Tree(Cotinus coggygria var. cinerea#br#)

Abstract

Abstract: The endophytic bacteria in leaf of Xiangshan smoke tree were isolated, screened and identified by the microbial culturedependent method combing with morphological observation, 16S rDNA amplification and phylogenetic analysis, indicated that the total 81 strains from the leaves were clustered into 7 genus: Erwinia and Shigella, Bacillus, Cohnella, Paenibacillus and Staphylococcus, and Curtobacterium belonging to Proteobacteria, Firmicutes and Actinobacteria, respectively. This is the first report about investigation on the endophytic bacteria in leaf of Xiangshan smoke tree using culture-dependent method.

Key words: Smoke tree, Leaf, Endophytic bacteria, Culture-dependent method

Zuo Shan, Liu Yang, Zhou Xiaohong3 Shen Yue, Li Zhe, Peng Cheng, Chen Liang, Cheng Chi. Isolation and Identification of Endophytic Bacteria in Leaf from Xiangshan Smoke Tree(Cotinus coggygria var. cinerea#br#)[J]. Biotechnology Bulletin, 2014, 0(1): 151-155.

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