Molecular Detection and Agronomic Traits Analysis of Insect-resistant Transgenic Maize Harboring Bt cry1Ah Gene

Abstract

Abstract: Bt cry1Ah gene which is a novel gene cloned by Chinese scientist was introduced into inbred line zong31 via agrobacterium-mediated transformation. An insect-resistant transgenic event was acquired by population screening of 1 764 transgenic plants. In this report we evaluated the transgenic event's insect resistance through laboratory and field bioassay and analyzed the foreign gene expression and the agronomic traits of hybrids generations. The results indicated that the transgenic event had high resistance to Asian corn boner(Ostrinia furnacalis), and it had no significant difference in the plant height, ear position, ear length and ear width between the transgenic event and non-transgenic plant. However, there were significantly shorter bald in transgenic event and thousand kernels weight and yield per plant were significantly higher than the control. It was extremely significantly difference in transgenic plant and non-transgenic plant under conditions of infestation by artificial simulation.

Key words: Transgenic, Naize Population, Screening, Nolecular detection Insect, Resistance Agronomic traits

Dai Jun, Li Xiuying, Zhu Li, Wang Hai, Zhang Jie, He Kanglai, Lang Zhihong, Huang Dafang. Molecular Detection and Agronomic Traits Analysis of Insect-resistant Transgenic Maize Harboring Bt cry1Ah Gene[J]. Biotechnology Bulletin, 2014, 0(5): 62-68.

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