A Study on Identification and Enzyme Characteristics of a Xylanase Producing Strain Isolated and Selected from the Soil in Alpine Meadow

Abstract

Abstract: A fungus strain has been isolated and selected from the soil in alpine meadow. Using gene sequences of rDNA-ITS analysis, flask liquid fermentation and traditional culture methods. Taxonomic position of strains were identificate, and xylanase characteristics and its main biological characteristics were also been investigated. The results showed that by rDNA-ITS sequence analysis, the fungus was identified as Saccharicola bicolor. S. bicolor can secrete xylanase, its enzyme activity is relatively stable under the condition, i.e., pH3.0-9.5, showed that xylanase secreted by S. bicolor have certain tolerance ability of alkaline conditions. The fungus grew rapidly under the condition, i.e., 35℃, showing different requirements for carbon and nitrogen resources, i.e., the order of carbon resources is maltose, starch, sucrose, dextrin, and glucose；the order of nitrogen resources is proteins, amine phosphate, amine sulfate, sodium nitrate, and urea.

Key words: Alpine, Neadow Xylanase, RDNA-ITS, PH Stability, Biological Characteristic

Lu Guangxin, Chen Xiurong, Wang Junbang, Wu Chu⁴. A Study on Identification and Enzyme Characteristics of a Xylanase Producing Strain Isolated and Selected from the Soil in Alpine Meadow[J]. Biotechnology Bulletin, 2014, 0(5): 162-178.

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