Isolation and Identification of a Root Rot Pathogen of Rehmannia glutinosa and Its Characterization

doi:10.13560/j.cnki.biotech.bull.1985.2019-0771

Abstract

Abstract: We aimed to isolate and identify the pathogens resulting in Rehmannia glutinosa root rot as well as study its pathogenicity and biological characteristics. In this study,streak plate method was used to isolate and purify the pathogens of rot root in R. glutinosa. ITS sequencing and genetic evolution analysis were used to identify the isolated strains. Inoculation experiment was used to confirm the pathogenic of the isolated potential pathogens. The growth test was performed to analyze physiological characteristics of the isolated stain. One of the obtained strains presented the colony morphology of orange color,the neat edge,the smooth and moist surface,and microscopic morphology with oval shape,suspected to be a fungus with buds. The strain was clustered together with the Rhodotorula paludigena at confidence of 91 and they displayed the close genetic relation. R. glutinosa roots inoculated with the strain and the incidence was 100% at day 20. The optimal growth temperature and pH of the strain was 28℃ and 5.0,respectively. The strain grew well in the medium with 0.5 g/L ferulic acid,vanillic acid and p-hydroxybenzoic acid,i.e.,the secretion in the root of R. glutinosa that was closely related to the root rot of R. glutinosa. It is preliminarily verified that R. paludigena DH5 may cause the root rot of R. glutinosa and well adapts to the rhizosphere environment containing a large content of phenolic compounds.

Key words: Rehamannia glutinosa, phytophthora root rot, Rhodotorula paludigena, phenolic acid, biological characteristics

WANG Ya-li, KANG Chun-xiao, YANG Chuan-zhen, WEI Yi-xuan, WANG Rui-fei, LI Ming-jun, YANG Qing-xiang. Isolation and Identification of a Root Rot Pathogen of Rehmannia glutinosa and Its Characterization[J]. Biotechnology Bulletin, 2020, 36(1): 37-44.

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