Abstract: Plant phosphate transporters（PTs）are active in the uptake of inorganic phosphate（Pi）from the soil and it's translocation within the plant. In this work, the key phosphate transporter gene OsPT8 in rice was transformed into tobacco（Yunyan 87）by Agrobacterium-mediated method. We set two treatments-normal P（1 mmol/L Pi）and low P（0.1 mmol/L Pi）by transgenic and wild-type tobacco as materials, detecting biomass and contents of P in shoots and roots, and analyzing the expression of phosphate transporter family gene（NtPT1 and NtPT2）. The results show that the biomass, the contents of total P and Pi of shoots and roots in transgenic line increased significantly compared with wild type under low Pi treatment, this data suggest that overexpression of OsPT8 can enhance the Pi deficiency tolerance of transgenic tobacco；the data of gene expression of NtPT1 and NtPT2 suggest that overexpression of OsPT8 enhance the Pi deficiency tolerance of transgenic tobacco caused by OsPT8 and other phosphate transporters.

Key words: Tobacco, Pi deficiency tolerance, Phosphate transporter, OsPT8