Isolation, Identification and Quorum Sensing of a Serratia spp.

Abstract

Abstract: One quorum sensing strain was isolated from spoilage Litopenaeus vannamei. Specie was determined by 16S rRNA gene analysis and classical tests, it can not produce red pigment on medium, named it Serratia marcescens AK1.Quorum sensing was detected by reporter strains and signal molecules types were identified by thin layer chromatography. Quantitative analysis of the signaling molecule secreted by AK1 during different growth stages. The result indicates strain AK1 can induce Chromobacterium violaceum CV026 and Agrobacterium tumefaciens A136 produce purple and blue color respectively. Thin layer chromatography showed that AK1 can produce C6-HSL and 3-oxo-C6-HSL types of signaling molecules with density-dependent. They reached the maximum in the late logarithmic phase.

Key words: Serratia marcescens, Identification, Quorum sensing, N-acyl-homoserine lactones

Zhang Caili, Zhu Suqin, Wang Ying, Sun Xiujiao, Zeng Mingyong. Isolation, Identification and Quorum Sensing of a Serratia spp.[J]. Biotechnology Bulletin, 2014, 0(7): 150-155.

References

[1] Fuqua WC, Winans SC, Greenberg EP. Quorum sensing in bacteria：the LuxR-LuxI family of cell density-responsive transcriptional regulators[J]. Journal of Bacteriology, 1994, 176（2）：269-275.
[2] Nealson KH, Platt T, Hastings JW. Cellular control of the synthesis and activity of the bacterial luminescent system[J]. Journal of Bacteriology, 1970, 104（1）：313-322.
[3] Davies DG, Parsek MR, Pearson JP, et al. The involvement of cell-to-cell signals in the development of a bacterial biofilm[J].Science, 1998, 280（5361）：295-298.
[4] Lee CY, Su SC, Liaw RB. Molecular analysis of an extracellular protease gene from Vibrio parahaemolyticus[J]. Microbiology, 1995, 141（10）：2569-2576.
[5] Miller MB, Skorupski K, Lenz DH, et al. Parallel quorum sensing systems converges to regulate virulence in Vibrio cholera[J]. Cell, 2002, 110（3）：303-314.
[6] Pongsilp N, Triplett EW, Sadowsky MJ. Detection of homoserine lactone-like quorum sensing molecules in Bradyrhizobium strains[J]. Current Microbiology, 2005, 51（4）：250-254.
[7] Miller MB, Bassler BL. Quorum sensing in bacteria[J]. Annual Reviews in Microbiology, 2001, 55（1）：165-199.
[8] Schauder S, Shokat K, Surette MG, et al. The LuxS family of bacterial autoinducers：biosynthesis of a novel quorum-sensing signal molecule[J]. Molecular Microbiology, 2001, 41（2）：463-476.
[9] Grimont PAD, Grimont F, Richard C, et al. Deoxyribonucleic acid relatedness between Serratia plymuthica and other Serratia species, with a description of Serratia odorifera sp. nov.（type strain：ICPB 3995）[J]. International Journal of Systematic Bacteriology, 1978, 28（4）：453-463.
[10] Rutherford ST, Bassler BL. Bacterial quorum sensing：its role in virulence and possibilities for its control[J]. Cold Spring Harbor Perspectives in Medicine, 2012, 2（11）：1-25
[11] Mclean KH, Winston MK, Fish L, et al. Quorum sensing and Chromobacterium violaceum：exploitation of violacein production and inhibition for the detection of N-acyl-homoserine lactones[J]. Microbiology, 1997, 143（12）：3703-3711.
[12] Zhu J, Beaber JW, Moré MI, et al. Analogs of the autoinducer 3-oxooctanoyl-homoserine lactone strongly inhibits activity of the TraR protein of Agrobacterium tumefaciens[J]. Journal of Bacteriology, 1998, 180（20）：5398-5405.
[13] Rice SA, Koh KS, Queck SY, et al. Biofilm formation and sloughing in Serratia marcescens are controlled by quorum sensing and nutrient cues[J]. Journal of Bacteriology, 2005, 187（10）：3477-3485.
[14] Chu W, Vattem DA, Maitin V, et al. Bioassays of quorum sensing compounds using agro bacterium tumefaciens and Chromobacterium violaceum［M] .Quorum Sensing. Humana Press, 2011：3-19.
[15] Tang K, Zhang Y, Yu M, et al. Evaluation of a new high-throughput method for identifying quorum quenching bacteria[J]. Scientific Reports, 2013, 3：1-9.
[16] 蔡妙英, 东秀珠. 肠杆菌科检索系统[J]. 微生物学通报, 1989, 5（19）：314-319.
[17] Bassler BL, Miller MB. Quorum Sensing［M] .The Prokaryotes. Springer Berlin Heidelberg, 2013：495-509.
[18] Horng YT, Deng SC, Daykin M, et al. The LuxR family protein SpnR functions as regulator of N-acyl-homoserine lactone dependent quorum sensing in Serratia marcescens[J].Molecular Microbiology, 2002, 45（6）：1655-1671.
[19] Van Houdt R, Givskov M, Michiels CW. Quorum sensing in Serratia[J]. FEMS Microbiology Reviews, 2007, 31（4）：407-424.
[20] Holly S, Matthew C, Lee E, et al. Phosphate availability regulates biosynthesis of two antibiotics, prodigiosin and carbapenem, in Serratia via both quorum-sensing-dependent and -independent pathways[J]. Molecular Microbiology, 2003, 47（2）：303-320.