Study on Aerosol Microbial Community in the Production Workshop of Morel Spawn

doi:10.13560/j.cnki.biotech.bull.1985.2022-0702

Abstract

Abstract:

This work is aimed to dissect the dynamic changes of aerosol microbial community in the production workshops of morel spawn and to find the environmental microbial factors affecting safe production of morel from the source of spawn production. High-throughput amplicon sequencing was used to monitor the aerosol microbial community in the inoculation room, original spawn cultivation room and cultivated spawn cultivation room during large-scale production of morel spawn. The results showed that the fungal diversity in the aerosol of the workshop increased significantly along with the increasing of amplification grade during morel-spawn production, while the bacterial diversity did not change significantly. The fungal community was dominated by Ascomycota, and the bacterial community was dominated by Proteobacteria, Firmicutes, and Actinomycetes. The bacterial communities of Pseudomonas and Lactobacillus were dominant in all types of workshops, and their proportions increased with the amplification level of morel strains. Phialemoniopsis sp. dominated the fungal communities in all types of workshops, with the proportions over 96%. There was a very high phylogenetic relationship between Phialemoniopsis sp. and Acremonium, a potential risk factor causing morel fructification failure, indicating that it may be one of the risk ecological factors affecting morel production. Our findings provide theoretical supplementation for environmental conditions required for safe production of morel spawn.

Key words: aerosol microorganism, morel, diversity, Pseudomonas, spawn production

YU Yang, LIU Tian-hai, LIU Li-xu, TANG Jie, PENG Wei-hong, CHEN Yang, TAN Hao. Study on Aerosol Microbial Community in the Production Workshop of Morel Spawn[J]. Biotechnology Bulletin, 2023, 39(5): 267-275.

Figures/Tables 6

Fig. 1 α-diversity estimation (A) and β-diversity estimation (B) based on principal coordinate analysis（PCoA）of the fungal and bacterial communities in aerosol of three types of workshops

Fig. 2 Composition of aerosol microbial communities

Fig. 3 Heatmap of fungal and bacterial genera (showing the top 20 prominent genera in the samples)

Fig. 4 Relative abundance of the major bacterial (A)and fungal (B) genera in the aerosol microbial communities of 3 workshops The same letters on the column indicate no significant difference（P>0.05）

Fig. 5 Relative abundances of fungal and bacterial (A, B) phyla and biomarker taxa (C, D) in the strain production workshop

Fig. 6 Phylogenetic analysis based on ITS sequence

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