Tag Selection of Tandem Affinity Purification and Its Application in Plant Protein-Protein Interactions

Abstract

Abstract:

Tandem affinity purification（TAP）is a technology for the purification of protein complex under the normal physiological conditions, which has been extensively utilized to identify protein-protein interactions（PPIs）and reveal interaction network of protein complexes. In recent years, TAP technology has been improving in the PPIs study, with the development of new labels, TAP method improvements and jointed use with other technologies associated. TAP technology is also being increasingly used in the studies of plant PPIs. This paper reviews the selection of TAP affinity tag and advance of research that TAP has been successful used in the study of plant PPIs.

Key words: Tandem affinity purification, Tag, Plant, Protein-protein interactions

Zhang Zhifei, Zhao Zhili, Wen Zhaozhu. Tag Selection of Tandem Affinity Purification and Its Application in Plant Protein-Protein Interactions[J]. Biotechnology Bulletin, 2014, 0(8): 23-27.

References

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