Endophytes Isolation and Broad-spectrum Antagonistic Bacterias Screening from Banana

Abstract

Abstract:

In order to determine the main distribution of endophytes and their broad-spectrum antimicrobial activity, endophytes were obtained from healthy and diseased tissues of two disease-resistant and one disease susceptible banana cultivars. Endophytes were separated from roots, corms, pseudostems, leaves and store in the ultra-low on Luria-Bertani（LB）, Yeast Extract with supplements（YE）, and Potato Dextrose Agar（PDA）strain store medium. Then screened broad-spectrum antagonistic bacteria which against Fusarium oxysporum f. sp. Cubense, Curvularia lunata, Curvularia fallax, Corynespora cassiicola（Berk & Curt）Wei, Alternaria musae, Deightoniella troulosa, Colletotrichum musae, Pestalogiopsis sp., Btoryosphaeria dothidea. Taxonomy identification of 041, 04-1, 19-1, 03A-1 was conducted by evaluating morphologic characteristics and 16S rDNA gene sequences for phylogenetic analysis. After purification, total of 438 endophytes were obtained. The total of isolates showed that we obtained 240 strains bacteria, followed by 142 strains actinomycetes, and 56 strains fungi. The richest number of endophytes that isolated from diseased NanTian banana cultivars（128）. Ten actinomyces and two bacterias were determined to possess antibiotic activity against Ten banana pathogens. Isolates 041 was the most effective and had 28.13±1.89 mm width of inhibition zone. Isolated 041, 04-1, 19-1, 034-1 were identified as Streptomyces misionensis.

Key words: Banana, Endophytes, Broad-spectrum antagonistic bacteria, Isolation

Wang Mengying, Zhou Dengbo, Jing Tao, Hu Yifeng, Gao Zhufen, Xie Qingyi, Zhang Xiyan, Qi Chunlin. Endophytes Isolation and Broad-spectrum Antagonistic Bacterias Screening from Banana[J]. Biotechnology Bulletin, 2014, 0(8): 138-145.

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