Isolation of Diesel Degrading Strain Acinetobacter sp. AK5 and Its Degrading Performance

Abstract

Abstract:

A diesel degradable bacterial strain was isolated from activated sludge and identified as Acinetobacter sp. AK5 through physiological, biochemical identification and 16S rDNA sequence analysis. Experiments of the different pH values, NaCl concentrations, culture time and diesel concentrations were detected to evaluate the diesel degradability by Acinetobacter sp. AK5. The results show that the optimal initial pH scope for the bacterial growth is from 5 to 9, the optimum NaCl concentrations is between 3% and 4%. When the diesel concentration is 5 g/L, the 7 d diesel degradation rate can reach 99%, while when the concentration of diesel is 20 g/L, 7 d diesel degradation rate can be 67%. The Acinetobacter sp. AK5 can grow well in artificial seawater medium and inorganic salt culture medium, therefore it has promising application prospect in seawater and freshwater oil pollution treatment.

Key words: Diesel, Degradation, Acinetobacter, Degradation rate

Xu Xiaoyu, Chen Jinghua. Isolation of Diesel Degrading Strain Acinetobacter sp. AK5 and Its Degrading Performance[J]. Biotechnology Bulletin, 2014, 0(8): 146-151.

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