Abstract: To optimize the docetaxel-containing liposome preparation technology, some liposome preparation methods were researched, such as reverse evaporation technique, high shear, film dispersion method, injection and multiple emulsion method. The single factor experiment was adopted to inspect the reverse evaporation technique and prescription preparation. The ideal combination of preparation and formulation were as follows：taxol-phospholipids=1∶25, phospholipid content 2.5%, cholesterol-phospholipids=1∶9. The reverse evaporation technique and the optimized preparation can be used to prepare paclitaxel liposome successfully.

Key words: Paclitaxel liposome, Preparation process , Single factor