A Rapid and Simple Method for DNA Extraction from Plant Leaves

Abstract

Abstract: In order to resolve the needs of PCR analysis of a large number of plants in study on molecular biology, we established a convenient and quick method for extraction of genomic DNA from plant leaves without grounding or organic reagents. The genomic DNA was isolated using TE buffer containing 1% beta-mercaptoethanol and crushed by a mini-beadbeater tissue homogenizer. Through the comparison of concentration and purity of genomic DNA and the results of PCR analysis, it indicated that the supernatant collected after quick frozen was suitable for PCR analysis. The whole protocol only takes 10-12 minutes, and it is a simple, efficient and economic method which needs less amount of samples. The genomic DNA of tobacco, rice, soybean, maize, rape and peanut extracted by this methord is suitable for PCR amplicafition. A 3 244 bp gene can be amplified and 4 new actin genes of peanuts are obtained using genomic DNA isolated by this method.

Key words: Genomic DNA, Rapid extraction , Cell disruption, PCR amplification

Chi Jing,Geng Lili, Gao Jiguo, Shu Changlong,Zhang Jie. A Rapid and Simple Method for DNA Extraction from Plant Leaves[J]. Biotechnology Bulletin, 2014, 0(9): 51-57.

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