The Research of an Improved Method for Protein Staining in Electrophoresis with Coomassie Brilliant Blue

Abstract

Abstract: In order to establish a convenient, sensitive, economic and Mass Spectrometry compatible staining method for protein polyacrylamide gel electrophoresis, herein, we established optimized staining methods based on Blue Silver Staining, by optimizing the gel immobilization time and buffer content. Comparison result indicated the method 1 keep the similar high sensitivity and well Mass Spectrometry compatible feature as Blue Silver Staining, what’ s more, is more easily conducted, less organic solvent consuming and have better correlation between protein amount and staining density.

Key words: Protein staining , Coomassie brilliant blue（CBB） , Sensitivity , Linear dynamic range（LDR） , Economic and environmental protection

Rao Weiqiao, Xiao Weimin, Zhang Jiyuan, Rao Yuan, Zi Jin. The Research of an Improved Method for Protein Staining in Electrophoresis with Coomassie Brilliant Blue[J]. Biotechnology Bulletin, 2014, 0(9): 58-64.

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