Abstract: In order to determine a set of SSR core primers fitting for pepper hybrids’ purity identification, 100 pepper（Capsicum）hybrids were analyzed by 17 SSR primers. According to their polymorpism and heterogosite rate, three primers Hpms1-214, Es395 and Hpms1-5 were determined as preferred core primers for pepper hybrid purity identification. Using three preferred core primers, 97 hybrids（accounting for 97%）have heterozygosis band type in at least one primer. Five primers Es330, Es363, Epms923, Es120 and Es64 were determined as candidate core primers for pepper hybrid purity identification. 14 varieties had specific primers within the 100 varieties. Primers with complementary band types were further screened for pepper purity identification.

Key words: Pepper germplasm , SSR , Hybrid , Purity identification, Core primers