Expression Pattern Analysis of Histone Methyltransferase Under Drought Stress in Hybrid Wheat

doi:10.13560/j.cnki.biotech.bull.1985.2021-1524

Abstract

Abstract:

The aim of this study is to screen the histone methyltransferases involved in the drought stress of wheat hybrids，and to provide functional markers for the improvement of stress resistance of two-line hybrid wheat. Screening of the total 175 TaHMT family genes in the wheat through the public databases on the website and the comparative genomics strategies，we obtained 8 candidate histone methyltransferases genes induced by drought stress（TaHMT21，TaHMT24，TaHMT31，TaHMT42，TaHMT49，TaHMT105，TaHMT143，TaHMT157）. The physical and chemical properties，protein structures，phylogenetic relationships，gene structures and conserved motifs of 8 TaHMTs were systematically analyzed via bioinformatics. Using drought treated hybrid wheat combinations（BS278*09YH91-5 and parental）as experimental materials，the candidate genes of 8 histone methyltransferase were verified via quantitative fluorescent PCR. It was found that their expressions were induced by drought stress，and the expression pattern presented mode of up-regulation first and then down-regulation with the drought stress time prolonged. The expressions of gene TaHMT21，TaHMT24 and TaHMT42 accorded with the phenotypes of hybrid combinations，indicating that they may regulate the drought resistance of hybrids. These results provide important clues to deeply study the function of wheat TaHMT family genes in the drought resistance of hybrids as well as also provide excellent stress resistance gene resources for wheat molecular breeding.

Key words: wheat, hybrid, TaHMT, bioinformatics analysis, drought stress

CHEN Jia-min, LIU Yong-jie, MA Jin-xiu, LI Dan, GONG Jie, ZHAO Chang-ping, GENG Hong-wei, GAO Shi-qing. Expression Pattern Analysis of Histone Methyltransferase Under Drought Stress in Hybrid Wheat[J]. Biotechnology Bulletin, 2022, 38(7): 51-61.

Figures/Tables 9

References 44

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引物名称Primer name	上游引物序列 Forward primer sequence（5'-3'）	下游引物序列 Reverse primer sequence（5'-3'）
actin	GTTGGTGATGAGGCCCAATC	GTGCTACACGGAGCTCATTG
TaHMT21	TGCCGCTGTGGTGTATACTG	CAGCCAAAAGACCCCATCCA
TaHMT24	TAAGGCTAGGGGCAACTCCT	CCATCCAACTCAGCAGTCGT
TaHMT31	GTTTGGTGGTGTGATGGTGC	TGCTGGAATGGTGTCCTTCG
TaHMT42	GGTCTGCGCAAGCAATTGAA	TCGTACAGGGCGTCGATTTC
TaHMT49	TTCTTCACGAGCAGGAAGGT	TTCCTCGGCAGTACTTGCTT
TaHMT105	AGACCTTAGAGAGCGGCGTA	ATGCTCATCCCCGAGAACAC
TaHMT143	TGATTGCAGCATGGACCAGT	GCAAACAGCCCCAAAGTACG
TaHMT157	CTACGGTTGCATCAAGCTCA	GCATCTCACAGTCCTCGTCA

引物名称Primer name	上游引物序列 Forward primer sequence（5'-3'）	下游引物序列 Reverse primer sequence（5'-3'）
actin	GTTGGTGATGAGGCCCAATC	GTGCTACACGGAGCTCATTG
TaHMT21	TGCCGCTGTGGTGTATACTG	CAGCCAAAAGACCCCATCCA
TaHMT24	TAAGGCTAGGGGCAACTCCT	CCATCCAACTCAGCAGTCGT
TaHMT31	GTTTGGTGGTGTGATGGTGC	TGCTGGAATGGTGTCCTTCG
TaHMT42	GGTCTGCGCAAGCAATTGAA	TCGTACAGGGCGTCGATTTC
TaHMT49	TTCTTCACGAGCAGGAAGGT	TTCCTCGGCAGTACTTGCTT
TaHMT105	AGACCTTAGAGAGCGGCGTA	ATGCTCATCCCCGAGAACAC
TaHMT143	TGATTGCAGCATGGACCAGT	GCAAACAGCCCCAAAGTACG
TaHMT157	CTACGGTTGCATCAAGCTCA	GCATCTCACAGTCCTCGTCA

基因名称Gene name	TaHMT21	TaHMT24	TaHMT31	TaHMT42	TaHMT49	TaHMT105	TaHMT143	TaHMT157
基因ID号Gene ID	TraesCS2A02G302100	TraesCS2A02G457100	TraesCS2B02G317900	TraesCS2D02G300800	TraesCS2D02G566400	TraesCS5B02G163000	TraesCS7B02G262900	TraesCS2A02G147100
染色体位置Chromosome location	2A：517 803 993-517 808 473（+）	2A：705 740 079-705 742 875（+）	2B：453 747 757-453 752 627（+）	2D：383 285 805-383 290 377（+）	2D：635 593 374-635 597 307（-）	5B：300 380 740-300 397 790（-）	7B：483 766 747-483 769 174（-）	2A：93 926 568-93 930 236（-）
亚细胞定位Subcellular localization	Nucleus	Nucleus	Nucleus	Nucleus	Nucleus	Nucleus	Nucleus	Nucleus
氨基酸数目Number of amino acids/aa	502	476	529	502	737	1086	390	601
分子量Molecular weight/kD	56 612.99	53 938.51	59 323.00	56 560.96	80 411.70	122 007.19	42 293.23	65 280.55
理论等电点Theoretical pI	4.77	5.47	4.78	4.80	7.09	7.59	4.44	4.48
负电荷残基数（Asp+Glu） Number of negatively charged residues（Asp+Glu）	83	64	84	82	84	146	65	99
正电荷残基数（Arg+Lys） Number of positively charged residues（Arg+Lys）	53	53	53	53	83	148	30	50
总原子数Total number of atoms	7 801	7 540	8 162	7 794	11 137	------	5 789	8 992
分子式Formula	C2458H3830N684O 805S24	C2392H3751N657O 716S24	C2572H4003N717O 843S27	C2458H3826N684O 802S24	C3478H5503N1035O 1080S41	------	C1833H2830N506O 594S26	C2859H4408N762O 937S26
不稳定指数Instability index	51.39	43.26	49.28	53.18	49.44	54.44	54.59	38.91
脂溶指数Aliphatic index	71.14	90.82	71.02	71.53	68.41	76.19	75.95	75.61
亲水性GRAVY	-0.631	-0.219	-0.584	-0.608	-0.511	-0.514	-0.301	-0.285

基因名称Gene name	TaHMT21	TaHMT24	TaHMT31	TaHMT42	TaHMT49	TaHMT105	TaHMT143	TaHMT157
基因ID号Gene ID	TraesCS2A02G302100	TraesCS2A02G457100	TraesCS2B02G317900	TraesCS2D02G300800	TraesCS2D02G566400	TraesCS5B02G163000	TraesCS7B02G262900	TraesCS2A02G147100
染色体位置Chromosome location	2A：517 803 993-517 808 473（+）	2A：705 740 079-705 742 875（+）	2B：453 747 757-453 752 627（+）	2D：383 285 805-383 290 377（+）	2D：635 593 374-635 597 307（-）	5B：300 380 740-300 397 790（-）	7B：483 766 747-483 769 174（-）	2A：93 926 568-93 930 236（-）
亚细胞定位Subcellular localization	Nucleus	Nucleus	Nucleus	Nucleus	Nucleus	Nucleus	Nucleus	Nucleus
氨基酸数目Number of amino acids/aa	502	476	529	502	737	1086	390	601
分子量Molecular weight/kD	56 612.99	53 938.51	59 323.00	56 560.96	80 411.70	122 007.19	42 293.23	65 280.55
理论等电点Theoretical pI	4.77	5.47	4.78	4.80	7.09	7.59	4.44	4.48
负电荷残基数（Asp+Glu） Number of negatively charged residues（Asp+Glu）	83	64	84	82	84	146	65	99
正电荷残基数（Arg+Lys） Number of positively charged residues（Arg+Lys）	53	53	53	53	83	148	30	50
总原子数Total number of atoms	7 801	7 540	8 162	7 794	11 137	------	5 789	8 992
分子式Formula	C2458H3830N684O 805S24	C2392H3751N657O 716S24	C2572H4003N717O 843S27	C2458H3826N684O 802S24	C3478H5503N1035O 1080S41	------	C1833H2830N506O 594S26	C2859H4408N762O 937S26
不稳定指数Instability index	51.39	43.26	49.28	53.18	49.44	54.44	54.59	38.91
脂溶指数Aliphatic index	71.14	90.82	71.02	71.53	68.41	76.19	75.95	75.61
亲水性GRAVY	-0.631	-0.219	-0.584	-0.608	-0.511	-0.514	-0.301	-0.285