Biotechnology Bulletin ›› 2014, Vol. 0 ›› Issue (9): 114-119.

Previous Articles     Next Articles

The Research on Mutagenesis Breeding of Phaeodactylum tricornutum and the Condition of EPA Producing

Liu Hongquan, Lin Xiaoyuan, Li Jieqiong, Pan Yihua   

  1. Guangxi National Oceanic and Biotechnology College,New Technology of Chemical and Biological Transformation Process of Guangxi Key Laboratory of University,Nanning 530006
  • Received:2014-03-07 Online:2014-09-15 Published:2014-09-07

Abstract: In order to obtain algal strains of high EPA production, Phaeodactylum tricornutum was mutated by 0.6% EMS. The mutant strain EP1 was isolated by single cell clone from 200 mutant clones. The EPA yield of EP1 was increased by 19.81% compared with the parent strain. The most suitable foster condition includes NaNO3 75 mg/L, pH7.5, day-night temperature 17-15℃, 12% inoculation quantity and training 7 days. The EPA yield of EP1 could reach 26.77 mg/g under the suitable foster condition. The results of subculture test showed that the mutant strain possesses good hereditary stability.

Key words: Phaeodactylum tricornutum , EMS mutagenesis , EPA