Studies on the Genetic Diversity of Polyscias by ISSR Marker

doi:10.13560/j.cnki.biotech.bull.1985.2015.01.017

Abstract

Abstract: In this study, nine species Polyscias leaves as experimental material, we analyzed the genetic diversity and relationship by ISSR markers, 9 stable and high polymorphism ISSR primers screened from 100 ISSR primers by optimized the system of PCR for Polyscias, a total of 70 bands were obtained by the polymorphic primers of 9 test specimens, including 61 polymorphic bands, the polymorphic percentage was 87.14%. The genetic similarity coefficients ranged from 0.346 9 to 0.816 3, suggesting that the substantial genetic divergence between some varieties. The clustering scheme showed that Polyscias had small differences of genetypes their genetic basis was comparatively narrow.

Key words: ISSR, Polyscias, foliage plants, genetic similarity coefficient, cluster analysis

Zhang Guowu, Liu Xuefeng, Zhou Xinghua, Huang Tao. Studies on the Genetic Diversity of Polyscias by ISSR Marker[J]. Biotechnology Bulletin, 2015, 31(1): 109-114.

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