Application and Research Progress of Inter-simple Sequence Repeat（ISSR）Marker in Medicinal Plants

doi:10.13560/j.cnki.biotech.bull.1985.2017.04.008

Abstract

Abstract: Inter-simple sequence repeat（ISSR）has been known as a novel molecular maker technology based on simple sequence repeat（SSR）. At present,the research and development of medicinal plants are facing the problems such as unclear drug substance,uncontrollable quality and exhausted resources. DNA molecular marker technology,especially ISSR,has provided a new solution for the above problems. Comparing the merits and demerits of several commonly used molecular markers,finally,we mainly introduced the principles and characteristics of DNA molecular marker ISSR applied in current research of the medicinal plants,and summarized the new advances on ISSR molecular technology and its application prospective in the genetic diversity and structure of medicinal plants,identification of germplasm resources,quality identification of traditional Chinese medicine,and genetic map,aiming at providing better reference for the development and utilization of medicinal plants.

Key words: ISSR, molecular makers, medicinal plant, quality identification, genetic map

REN Meng-yun, CHEN Yan-jun, ZHANG Dun, DU Le-shan, LIU Fang, GUAN Xiao, ZHANG Yin-dong. Application and Research Progress of Inter-simple Sequence Repeat（ISSR）Marker in Medicinal Plants[J]. Biotechnology Bulletin, 2017, 33(4): 63-69.

References

[1] Bremness L. Herbs[M]. London：Dorling Kindersley, 1994.
[2] 臧敏, 邱筱兰, 姚丽芳, 等. 江西三清山野生药用植物资源分析[J]. 江苏农业科学, 2015, （2）：358-361.
[3] 高蕊. 丹东地区野生药用植物资源多样性分析[J]. 防护林科技, 2016, （8）：58-61.
[4] Li TSC. Chinese & Related North American Herbs：Phytopharmacology & Therapeutic Values[M]. Boca Raton：CRC press, 2016.
[5] 张影波, 袁媛, 庞玉新, 等. 艾纳香遗传多样性的SRAP和AFLP对比分析[J]. 南京农业学报, 2016, 47（8）：1261-1267.
[6] Fu PC, Zhang YZ, Ya HY, et al. Characterization of SSR genomic abundance and identification of SSR markers for population genetics in Chinese jujube（Ziziphus jujuba Mill. ）[J]. Peer J, 2016, 4：e1735.
[7] Nagaoka T, Ogihara Y. Applicability of intersimple sequence repeat polymorphisms in wheat for use as DNA markers in comparison to RFLP and RAPD markers[J]. Theoretical and Applied Genetics, 1997, 94（5）：597-602.
[8] 张民照, 崔桂山, 王进忠, 等. 基于RAPD改进的分子标记技术[J]. 应用昆虫学报, 2008, 45（6）：979-983.
[9] 蒋彩虹, 王元英, 孙玉合. SSR和ISSR标记技术应用进展[J]. 中国烟草科学, 2007, 28（2）：1-5.
[10] Ghislain M, Spooner DM, Rodríguez F, et al. Selection of highly informative and user-friendly microsatellites（SSRs）for genotyping of cultivated potato[J]. Theoretical & Applied Genetics, 2004, 108（5）：881-890.
[11] 汪由, 吴禹, 李兆波, 等. SSR分子标记及其在农作物育种中的应用[J]. 农业科技与装备, 2011, （2）：17-21.
[12] John BB, Joseph OO, Gbolagade JS. Molecular variability of Celosia argentea using amplified fragment length polymorphism（AFLP）marker[J]. Molecular Plant Breeding, 2016, 26（7）：1-6.
[13] 王华新, 王永勤, 曹家树, 等. 白菜雄性不育相关新基因BcMF1的分离及特征分析[J]. 中国农业科学, 2008, 41（4）：1119-1127.
[14] Hegab AA, Fahmy MM, Mahdy OA, et al. Parasitological and molecular identification of Theileria Species by PCR-RFLP Method in Sheep, Egypt. [J], Int J Adv Res Biol Sci, 2016, 3（7）：48-55.
[15] Outram MA, Jones EE, Jaspers MV, et al. Development of a PCR-RFLP method to distinguish species within the Ilyonectria macrodidyma complex[J]. New Zealand Plant Protection, 2014, 67：151-156.
[16] Pharmawati M, Imaniar EF. PCR-RFLP and Sequencing of trnS/trnfM Fragment of Enhalus acoriodes from Sanur Coastal Waters, Bali, Indonesia：A Preliminary Study[J]. Journal of Tropical Life Science, 2016, 6（2）：118-122.
[17] 朱国锋, 吴兰, 李思光. RFLP技术在湖泊微生物多样性研究中的应用[J]. 环境科学与技术, 2008, 31（11）：9-12.
[18] 匡猛, 杨伟华, 许红霞, 等. 分子标记技术在棉花品种鉴定上的研究进展[J]. 棉花学报, 2009, 21（4）：330-334.
[19] Guan Z, Zhang L, Song M, et al. Genetic relationship on several medicinal plants in Marsdenia from Yunnan in ISSR marker[J]. China journal of Chinese materia medica, 2012, 37（11）：1550-1552.
[20] Rampersad SN. Genetic structure of colletotrichum gloeosporioides sensu lato isolates infecting Papaya inferred by multilocus ISSR markers[J]. Phytopathology, 2013, 103（2）：182-189.
[21] 王照兰, 杨持, 赵丽丽, 等. 扁蓿豆不同品系ISSR标记遗传差异和遗传多样性[J]. 中国草地学报, 2010, 32（1）：11-17.
[22] Zietkiewicz E, Rafalski A, Labuda D. Genome fingerprinting by simple sequence repeat（SSR）-anchored polymerase chain reaction amplification[J]. Genomics, 1994, 20（2）：176-183.
[23] Gupta M, Chyi YS, Romero-severson J, et al. Amplification of DNA markers from evolutionarily diverse genomes using single primers of simple-sequence repeats[J]. Theoretical and Applied Genetics, 1994, 89：998-1006.
[24] Zhan S, Guo Q, Li M, et al. Disruption of an N-acetyltransferase gene in the silkworm reveals a novel role in pigmentation[J]. Development, 2010, 137（23）：4083-4090.
[25] Azevedo AL, Costa, PP, Machado MA. , et al. High degree of genetic diversity among genotypes of the forage grass Brachiaria ruziziensis（Poaceae）detected with ISSR markers[J]. Genetics and Molecular Research, 2011, 10（4）：3530-3538.
[26] Mcgregor CE, Lambert CA, Greyling MM, et al. A comparative assessment of DNA fingerprinting techniques（RAPD, ISSR, AFLP and SSR）in tetraploid potato（Solanum tuberosum L. ）germplasm[J]. Euphytica, 2012, 113（2）：135-144.
[27] Wolff K, Zietkiewicz E, Hofstra H. Identification of chrysanthemum cultivars and stability of DNA fingerprint patterns[J]. Theoretical and Applied Genetics, 1995, 91（3）：439-447.
[28] 钱韦, 葛颂, 洪德元. 采用RAPD和ISSR标记探讨中国疣粒野生稻的遗传多样性[J]. 植物学报, 2000, 42（7）：741-750.
[29] Guo GY, Yang RW, Ding CB, et al. Phylogenetic relationships between Leymus and related diploid Triticeae species revealed by ISSR markers[J]. Biologia, 2014, 69（8）：986-993.
[30] 陈名红, 熊华斌, 李成云. 分子标记在百合属植物遗传多样性研究中的应用[J]. 生物技术通报, 2011, （12）：65-69.
[31] Strasburg JL, Sherman NA, Wright KM, et al. What can patterns of differentiation across plant genomes tell us about adaptation and speciation?[J]. Philosophical Transactions of the Royal Society B Biological Sciences, 2012, 367（1587）：364-373.
[32] Yu HH, Yang ZL, Sun B, et al. Genetic diversity and relationship of endangered plant Magnolia officinalis（Magnoliaceae）assessed with ISSR polymorphisms[J]. Biochemical Systematics & Ecology, 2011, 39（2）：71-78.
[33] 王印肖, 徐秀琴, 韩宏伟. 分子标记在品种鉴定中的应用及前景[J]. 河北林业科技, 2006, （B09）：46-49.
[34] Medhi K, Sarmah DK, Deka M, et al. High gene flow and genetic diversity in three economically important Zanthoxylum Spp. of Upper Brahmaputra Valley Zone of NE India using molecular markers[J]. Meta Gene, 2014, （2）：706-721.
[35] Phong DT, Hien VT, Thanh TT, et al. Comparison of RAPD and ISSR markers for assessment of genetic diversity among endangered rare Dalbergia oliveri（Fabaceae）genotypes in Vietnam[J]. Genetics and Molecular Research, 2011, 10（4）：2382-2393.
[36] Zhang L, Lu S, Sun D, et al. Genetic variation and geographical differentiation revealed using ISSR markers in tung tree, Vernicia fordii[J]. Journal of Genetics, 2015, 94：5-9.
[37] Givnish TJ, Bean GJ, Ames M, et al. Phylogeny, floral evolution, and inter-island dispersal in Hawaiian Clermontia（Campanulaceae）based on ISSR variation and plastid spacer sequences[J]. PLoS One, 2013, 8（5）：e62566.
[38] 张颖娟, 王斯琴花. 不同苜蓿种质材料的ISSR分析及遗传多样性研究[J]. 中国草地学报, 2014, 36（3）：35-39.
[39] 李江伟, 杨琴军, 刘秀群, 等. 台湾杉遗传多样性的ISSR分析[J]. 林业科学, 2014, 50（6）：61-66.
[40] 詹羽姣, 盛萍, 姚蓝, 等. 新疆贝母属8种药用贝母遗传多样性ISSR分析[J]. 中国野生植物资源, 2015, 34（4）：1-6.
[41] 王果平, 樊丛照, 李晓瑾, 等. 基于ISSR的新疆贝母属植物遗传多样性研究[J]. 中草药, 2013, 44（7）：887-890.
[42] Liu C, Xue GP, Cheng B, et al. Genetic diversity analysis of Capparis spinosa L. populations by using ISSR markers[J]. Genetics & Molecular Research, 2015, 14（4）：16476-16483.
[43] 周文平, 张惠娟, 王亚飞, 等. 秦艽组6种植物的ISSR扩增结果分析[J]. 中药材, 2015（7）：1375-1378.
[44] 任风鸣, 金江群, 焦雁翔, 等. 中药金钱草种质资源的ISSR遗传多样性研究[J]. 中国药学杂志, 2015, 50（15）：1277-1281.
[45] 卢家仕, 卜朝阳, 吕维莉, 等. 不同产地石斛属种质资源的ISSR遗传多样性分析[J]. 中草药, 2013, 44（1）：96-100.
[46] 阳翠, 刘萍, 刘姣蓉, 等. 苦豆子ISSR标记的遗传多样性分析[J]. 中草药, 2013, 44（10）：1323-1327.
[47] Safaei M, Sheidai M, Alijanpoor B, et al. Species delimitation and genetic diversity analysis in Salvia with the use of ISSR molecular markers[J]. Acta Botanica Croatica, 2016, 75（1）：42-52.
[48] 于华会, 杨志玲, 杨旭, 等. 药用植物种质资源ITS序列研究进展[J]. 中草药, 2010, 41（3）：491-496.
[49] 周晔, 唐铖, 安适之, 等. ISSR法鉴定中药玉竹与小玉竹[J]. 中医药学报, 2006, 34（5）：7-9.
[50] 周晔, 王润玲, 唐铖, 等. ISSR法鉴定中药黄精与卷叶黄精[J]. 天津医科大学学报, 2006, 12（2）：178-180.
[51] 潘清平, 周日宝, 陈玉秀, 等. 玉竹不同品种的ISSR分子鉴定[J]. 中国现代中药, 2008, 10（10）：28-30.
[52] Pongsrila P, Chomnawang P, Srisamoot N, et al. DNA finger printing analysis of fourteen species of orchids using ISSR technique[J]. KKU Res J, 2014, 19（Suppl）：210-216.
[53] 毕泉鑫, 金则新, 李钧敏, 等. 枫香自然种群遗传多样性的ISSR分析[J]. 植物研究, 2010（1）：120-125.
[54] 刘燕, 张道远, 杨红兰. 准噶尔无叶豆5个自然居群ISSR遗传变异的空间自相关分析[J]. 生物多样性, 2010, 18（2）：129-136.
[55] Pivorien O, Akinskien IP, Brazauskas G, et al. Inter-simple sequence repeat（ISSR）loci mapping in the genome of perennial ryegrass[J]. Biologija, 2008, 54（1）：17-21.
[56] 战晴晴, 隋春, 魏建和, 等. 利用ISSR和SSR分子标记构建北柴胡遗传图谱[J]. 药学学报, 2010（4）：517-523.
[57] 王述彬, 吴小丽, 刘金兵, 等. 辣椒抗黄瓜花叶病毒（CMV）基因的ISSR标记[J]. 分子植物育种, 2009, 7（3）：569-572.
[58] 杨红兵, 崔光红, 詹亚华, 等. 湖北恩施产厚朴ISSR指纹图谱构建[J]. 中药材, 2009, 32（1）：19-22.
[59] Feng S, Zhao H, Lu J, et al. Preliminary genetic linkage maps of Chinese herb Dendrobium nobile and D. moniliforme[J]. Journal of Genetics, 2013, 92（2）：205-212.
[60] Gilbert JE, Lewis RV, Wilkinson MJ, et al. Developing an appropriate strategy to assess genetic variability in plant germplasm collections[J]. Theoretical and Applied Genetics, 1999, 98（6）：1125-1131.
[61] 李海生. ISSR分子标记技术及其在植物遗传多样性分析中的应用[J]. 生物学通报, 2004, 39（2）：19-21.
[62] Yang CL, Wu XP, Chen BZ, et al. Comparative analysis of genetic polymorphisms among Monascus strains by ISSR and RAPD markers[J]. Journal of the Science of Food and Agriculture, 2017, 97（2）：636-640.
[63] Haritha G, Sudhakar T, Chandra D, et al. Informative ISSR Markers Help Identify Genetically Distinct Accessions of Oryza rufipogon for Use in Yield Improvement[J]. Rice Science, 2016, 23（5）：225-241.
[64] Le NT, Mien NT, Tien TV, et al. Genetic diversity of Tsai in North Vietnam detected by inter simple sequence repeat（ISSR）markers[J]. Biotechnology & Biotechnological Equipment, 2016, 30（3）：1-6.
[65] Parveen S, Shahzad A. Application of molecular markers in medicinal climbers[M]. Berlin：Springer International Publishing, 2016：415-425.
[66] Purkayastha J. Bioprospecting of Indigenous bioresources of North-East India[M/OL]. Springer Nature, 2016, doi:10. 1007/978-981-10-0620-3.
[67] 刘靖宇, 宋秀高, 叶夏, 等. 香菇菌株遗传多样性ISSR、RAPD和SRAP综合分析[J]. 食用菌学报, 2011, 18（3）：1-8.