Research of PKS2 Interaction with CAM4 in Arabidopsis thailana

doi:10.13560/j.cnki.biotech.bull.1985.2015.02.013

Abstract

Abstract: Blue-light receptors phototropin （PHOT）mediate a wide set of physiological and developmental responses. As some PHOT downstream signal transduction components have been identified in Arabidopsis by genetic analyses, we have found that PKS1 of Phytochrome kinase substrate PKS family interacts with members of calmodulin （CAM）, which are Ca²⁺ binding protein. In order to address the issue of how PKS2 interacts with CAM4, firstly cDNA full length sequences of PKS2 and CAM4 were obtained by RT-PCR technique. Then, it was confirmed that PKS2 could be interact with CAM4 by tests of yeast two-hybrid system and bimolecular fluorescence complementation. These date could contribute to enrich relation between PKS and CAM, which would provide base for further uncovering PHOT functions.

Key words: Arabidopsis thaliana, phototropin, phytochrome kinase substrate, calmodulin

Qiao Xinrong, Duan Hongbin, Liu Zhuming, Zhao Fuan. Research of PKS2 Interaction with CAM4 in Arabidopsis thailana[J]. Biotechnology Bulletin, 2015, 31(2): 91-96.

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