Multiplex Automated Genome Engineering

doi:10.13560/j.cnki.biotech.bull.1985.2015.06.009

Abstract

Abstract: Genome editing is widely used in genome engineering research and site-specific nuclease technologies and CRISPR/Cas system focus on single gene editing. Owing to the huge size of genome, there are some limitations on the applications of these technologies. Multiplex Automated Genome Engineering(MAGE)is a new, fast and efficient genome editing technology, which can operate multiple genes simultaneously, and be used in knockout and replacement of Escherichia coli genes. This review illustrates the recent advances in the theory, operation protocol and technological innovation of MAGE, its application and development trend were also discussed.

Key words: multiplex automated genome engineering, genome editing, genome engineering, Escherichia coli

Li Dan, Gao Haijun. Multiplex Automated Genome Engineering[J]. Biotechnology Bulletin, 2015, 31(6): 60-66.

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