Screening，Identification and Potassium-dissolving Characteristics of Potassium-dissolving Actinomycete in Banana Rhizosphere Soil

doi:10.13560/j.cnki.biotech.bull.1985.2015.06.020

Abstract

Abstract: According to the characteristics and mechanism of potassium-dissolving by potassium-dissolving bacteria, strains of the highly efficient and stable potassium-dissolving bacteria were isolated from the banana rhizosphere soil. Firstly by primary screening in potassium-dissolving separation culture medium and screening culture medium, a number of highly efficient potassium-dissolving bacteria were obtained. Then identifying the strain types and determining the potassium-dissolving ratio in laboratory, highly efficient strains of potassium-dissolving actinomycetes were gained. Finally, based on the morphological, physiological and biochemical characteristics and 16S rRNA gene sequence analysis, the strains were identified, the potassium-dissolving ratios of them were measured under different conditions, and the potassium-dissolving characteristics were studied. After identifying the isolated 16 strains of efficient potassium-dissolving bacteria and measuring their potassium-dissolving ratios, finally a stable potassium-dissolving Streptomycete M3-4 was obtained. The potassium-dissolving ratio reached the highest under the conditions: 120 h, 30℃, pH=6, the medium potassium mineral content was 5 g, shaking speed in 250 r/min, maltose as carbon source, and peptone as nitrogen sources. The strain is Streptomyces shaanxiensis and potassium-dissolving ratio is about 20%.

Key words: banana rhizosphere soil, actinomycete, potassium-dissolving ratio

Chen Yufeng, Ke Chunliang, Zhou Dengbo, Gao Zhufen, Qi Chunlin, Zhang Xiyan. Screening，Identification and Potassium-dissolving Characteristics of Potassium-dissolving Actinomycete in Banana Rhizosphere Soil[J]. Biotechnology Bulletin, 2015, 31(6): 129-137.

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