Screening of Actinomycetes Against Phytophthora Root Rot of Soybean and Its Growth Promotion and Disease Control

doi:10.13560/j.cnki.biotech.bull.1985.2020-0092

Abstract

Abstract:

To screen the strain with good antagonistic effect against the phytophthora root rot of soybean,actinomycete XS1-5 with significant antagonism was isolated from soybean rhizosphere soil by agar block method and mycelia growth assay while taking the Phytophthora sojae of phytophthora root rot pathogen. Strain XS1-5 was identified as Streptomyces albosporeus based on morphological,physiochemical characters as well as molecular methods. The pot experiment was carried out with soybean seeds coated with different spore concentrations of the XS1-5. The results showed the application of the XS1-5 significantly promoted soybean growth,and increased chlorophyll content,root vitality and the protective enzymes in the leaf and root of soybean. When spore concentration was 1.0×10 ⁸ CFU/g,the plant height,root length,fresh weight and dry weight increased by 19.92%、28.26%、87.40% and 34.51%;meanwhile,chlorophyll content（7.53 mg/L）by 50.6% and root vitality（685.41 μg/g·h）by 2.4 folds increased,compared with the control,respectively,as well as the CAT,SOD and POD activity of leaf increased significantly by 158.86%,459.71% and 135.45%. The above results indicate that the antagonistic strain XS1-5 screened in this study has a certain disease-control and growth-promoting effect on soybean.

Key words: phytophthora root rot, actinomycetes, growth promotion, disease control

ZHANG Hong-yan, GAO qing, ZHANG Lin-yuan, LIN Guo-li, LI Ru-lian. Screening of Actinomycetes Against Phytophthora Root Rot of Soybean and Its Growth Promotion and Disease Control[J]. Biotechnology Bulletin, 2020, 36(10): 25-31.

Figures/Tables 9

References 23

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菌株编号	D/mm	T	孢子丰富
XS1-5	20.5±3.4a	+++	+++
GF1	15.2±3.1a	+++	+++
GT26	7.5±2.6c	+++	+
YU41	9.3±2.9bc	+++	+++
YU54	10.6±3.6bc	+++	+++
GF20	8.9±2.8c	+++	+++
X9	15.7±4.1a	+++	++

菌株编号	D/mm	T	孢子丰富
XS1-5	20.5±3.4a	+++	+++
GF1	15.2±3.1a	+++	+++
GT26	7.5±2.6c	+++	+
YU41	9.3±2.9bc	+++	+++
YU54	10.6±3.6bc	+++	+++
GF20	8.9±2.8c	+++	+++
X9	15.7±4.1a	+++	++

菌株	稀释度	48 h	72 h	96 h	120 h	144 h	168 h
XS1-5	1:1	15.2±3.7b	57.3±9.9a	76.6±6.7a	86.6±6.0a	90.9±8.0a	89.4±4.8a
	1：10	12.5±2.5c	35.5±1.2bc	51.5±7.7b	69.7±9.1b	77.9±5.9b	72.3±9.2b
	1:100	12.4±0.5c	33.7±2.4c	42.4±3.3c	54.1±6.2cd	67.5±6.8c	40.1±9.1f
GF1	1:1	20.9±6.2a	39.4±9.9b	42.8±6.2c	59.6±8.4c	69.2±9.2c	67.0±8.0c
	1:10	26.4±0.9a	36.1±2.3bc	43.1±6.7c	50.8±9.0e	56.5±6.5d	53.1±5.3d
	1:100	20.5±1.0a	25.3±3.4d	28.1±4.6e	35.2±8.3g	50.9±5.5e	42.7±6.1e
X9	1:1	13.8±0.1c	25.4±0.2d	49.1±2.0b	69.4±5.1b	57.5±4.9d	45.8±3.0e
	1:10	23.2±2.2a	26.7±2.6d	40.5±6.8cd	52.1±7.7cd	44.3±8.2f	36.5±5.0g
	1:100	15.2±2.0b	20.0±3.6e	31.2±6.1e	40.0±6.9f	45.3±6.9f	40.5±6.3f

菌株	稀释度	48 h	72 h	96 h	120 h	144 h	168 h
XS1-5	1:1	15.2±3.7b	57.3±9.9a	76.6±6.7a	86.6±6.0a	90.9±8.0a	89.4±4.8a
	1：10	12.5±2.5c	35.5±1.2bc	51.5±7.7b	69.7±9.1b	77.9±5.9b	72.3±9.2b
	1:100	12.4±0.5c	33.7±2.4c	42.4±3.3c	54.1±6.2cd	67.5±6.8c	40.1±9.1f
GF1	1:1	20.9±6.2a	39.4±9.9b	42.8±6.2c	59.6±8.4c	69.2±9.2c	67.0±8.0c
	1:10	26.4±0.9a	36.1±2.3bc	43.1±6.7c	50.8±9.0e	56.5±6.5d	53.1±5.3d
	1:100	20.5±1.0a	25.3±3.4d	28.1±4.6e	35.2±8.3g	50.9±5.5e	42.7±6.1e
X9	1:1	13.8±0.1c	25.4±0.2d	49.1±2.0b	69.4±5.1b	57.5±4.9d	45.8±3.0e
	1:10	23.2±2.2a	26.7±2.6d	40.5±6.8cd	52.1±7.7cd	44.3±8.2f	36.5±5.0g
	1:100	15.2±2.0b	20.0±3.6e	31.2±6.1e	40.0±6.9f	45.3±6.9f	40.5±6.3f

XS1-5	气生菌丝	基内菌丝	可溶性色素
蔗糖硝酸盐琼脂	无	无色	无
葡糖天冬素琼脂	白色	红色	无
淀粉琼脂	无	微红色	无
甘油苹果酸钙琼脂	白色	粉色	无
营养琼脂	无	乳脂色	无