Abstract: Using the fermented liquid and mycelium extract of actinomycetes FX-139 isolated from ginseng rhizosphere as the donor, the effects of it on the growth and ability of inducing defense enzyme activity of ginseng callus were studied. The results showed that: （1）The treatment of FX-139 fermented liquid and mycelium extract at the concentration of 20 mg/L had the strongest promoting effect on Ginseng callus growth, increasing 68.96% and 51.60% higher than that in the control, i.e., there was significant difference. （2）There was an active peak of PAL, POD and PPO in 21 d, 14 d and 28 d respectively under the treatment with water saturation n-butyl alcohol extract of fermented liquid, it raised 1.29 times, 2.5 times, and 2.12 times against the control group respectively. There was an active peak of PAL, POD and PPO in 21 d, 14 d and 14 d respectively under the treatment with acetone extract of mycelium, it raised 1.9 times, 2.6 times, and 1.4 times against the control group respectively. （3）The change of defense enzyme activities in ginseng callus was detected. PAL, POD and PPO enzyme activity were obviously changed with the treatment of fermented liquid and mycelium extract. Overall, Ginseng callus defense responses could be induced by fermented liquid and mycelium extract of FX-139. Fermented liquid extract could effectively induced POD and PPO, and mycelium extract could effectively induced PAL, POD and PPO.

Key words: actinomycetes, Ginseng callus, PAL, POD, PPO