The Next Generation Sequencing Technology and Its Applications

doi:10.13560/j.cnki.biotech.bull.1985.2015.11.003

Abstract

Abstract: DNA sequencingtechnology isan important research method inlifescience. Since thebirth of theSanger sequencing technology, DNA sequencingtechnologyhas experienced three generationsof change, resulting the secondgeneration to the fourth generation sequencingtechnology. DNA sequencing not only has been improving its productivity in an exponential growth rate but also been evolving into a new technological territories toward physical disciplines of nanotechnology. Next generation sequencingthat has landmark significance oflife sciences, can improve researches in the genome, transcriptome and epigenome. This review analyzes technical characteristics of the next generation sequencers and provide prospective insights into their future development and applications.

Key words: next generation sequencing, sequence by synthesis, single molecule sequencing, nanopore, applications

Tian Li, Zhang Ying, Zhao Yunfeng. The Next Generation Sequencing Technology and Its Applications[J]. Biotechnology Bulletin, 2015, 31(11): 1-8.

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