The Knockout of Gene ptsG of Recombinant Escherichia coli Producing D-lactic Acid and the Simultaneous Fermentation of Mixed Sugars

doi:10.13560/j.cnki.biotech.bull.1985.2015.12.032

Abstract

Abstract:

In order to construct a recombinant engineering Escherichia coli strain that yields D-lactic acid in the simultaneous efficient fermentation of pentose and hexose, having an engineering E. coli JH13 that efficiently ferment the pentose to produce D-lactic acid as an original strain, a glucose transmembrane transporter gene ptsG was knocked out by the technique of Red homologous recombination. The fermentative results showed that in the 10% mixed sugars(5% glucose and 5% xylose), the ptsG-deleted strain E. coli JH15 simultaneously utilized pentose and hexose to complete the fermentation；however, the control strain started to utilize the xylose only after glucose was consumed up, and 18 g/L xylose still remained after the fermentation completed. The production of D-lactic acid by JH15 reached 83.04 g/L, and 25.86% higher than that by control strain JH13. The JH15 as a E. coli strain of producing D-lactic acid during simultaneous fermentation with mixed sugars, its construction provides a reference for producing the D-lactic acid in fermentation while utilizing the low-cost hydrolyzed components of lignocelluloses materials as raw material.

Key words: recombinant Escherichia coli, D-lactic acid, ptsG, Red homologous recombination, mixed sugars

Ding Xiaoyun, Gu Jianjian, Wang Yongze, Zhao Jinfang, Wang Jinhua, Zhao Xiao. The Knockout of Gene ptsG of Recombinant Escherichia coli Producing D-lactic Acid and the Simultaneous Fermentation of Mixed Sugars[J]. Biotechnology Bulletin, 2015, 31(12): 221-226.

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