The Optimization of Flask Fermentation Conditions for the Production of Extracellular Lipase from Aspergillus niger

doi:10.13560/j.cnki.biotech.bull.1985.2015.12.033

Abstract

Abstract: Aspergillus niger G62s is a genetically stable strain of producing high-yield lipase. This work is to have statistic optimization for the flask fermentation conditions of G62s aiming at increasing the capacity of strain yielding lipase. Firstly, using single-factor test, the effects of these 4 parameters, i.e., the inoculation amount, working volume, culture temperature and culture time, on the lipase yield were studied. Then the key parameters of fermentation conditions were statistically optimized through the response surface methodology(RSM)with 4 factors in 3 levels. The optimal conditions were 1.9% inoculum size in 56 mL working volume(in 500 mL flasks)at 30^oC for 75 h. Using these conditions, the activity of lipase reached 212 9±39.9 U/mL, increased 16.7% comparing to that of the initial fermentation conditions. In conclusion, based on the single-factor test of 4 fermentation factors affecting the lipase yield of G62s, optimization by RSM significantly increased the yield of the target strain.

Key words: Aspergillus niger, lipase, single-factor test, response surface methodology, optimization of fermentation conditions

Zhang Qian, Jia Jia, Lin Zhi, Yang Xiaofeng, Guo Hongtao, Wang Jianying, Carol Sze Ki Lin. The Optimization of Flask Fermentation Conditions for the Production of Extracellular Lipase from Aspergillus niger[J]. Biotechnology Bulletin, 2015, 31(12): 227-233.

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